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Table 1.

Nuclear translocation of endogenous MRTF A after force application

Cells exhibiting nuclear staining for endogenous MRTF A (%)
Time (minutes) Untreated Latrunculin B (1 μM, 30 minutes before force treatment) Y27632 (10 μM, 30 minutes before force treatment)
0   6±2   5±2   4±2  
15   12±3   6±2   7±3  
30   27±5   9±3   10±4  
45   48±6   11±4   12±4  
Cells exhibiting nuclear staining for endogenous MRTF A (%)
Time (minutes) Untreated Latrunculin B (1 μM, 30 minutes before force treatment) Y27632 (10 μM, 30 minutes before force treatment)
0   6±2   5±2   4±2  
15   12±3   6±2   7±3  
30   27±5   9±3   10±4  
45   48±6   11±4   12±4  

Cells were incubated with collagen beads and force was applied for 0, 15, 30 or 45 minutes. Cells were fixed and stained for endogenous MRTF A using a polyclonal antibody generously donated by H. Nakano (Juntendo University School of Medicine, Tokyo, Japan). Percentage of cells that exhibited nuclear staining for endogenous MRTF A was evaluated by fluorescence microscopy. A significantly higher percentage of MRTF-A-stained nuclei was found 15, 30 or 45 minutes after force application in untreated (control cells) compared with those treated with latrunculin B or Y27632 (P<0.05)

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