Analyses of CBs in transiently transfected COS cells with SMN mutants
| . | Proportion of cells with . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | P80-CB (%) . | P80-positive FP foci (%) . | Endogenous SMN foci (%) . | Endogenous SMN-positive FP foci (%) . | TMG foci (%) . | TMG-positive FP foci (%) . | U2B” foci (%) . | U2B”-positive FP foci (%) . | |||||||
| Untransfected | 75 (n=717) | - | 71 (n=704) | - | 51 (n=205) | - | 39 (n=200) | - | |||||||
| Constructs* | |||||||||||||||
| SMN† | 100 (n=79) | 100 | nd | nd | 46 (n=100) | 46 | 32 (n=412) | 32 | |||||||
| SMNΔN86† | 95 (n=83) | 100 | 88 (n=100) | 88 | 9 (n=109) | 9 | 5 (n=221) | 5 | |||||||
| SMNΔex7† | 100 (n=132) | 100 | nd | - | 5 (n=183) | 5 | 6 (n=217) | 6 | |||||||
| SMNΔC40 | 80 (n=106) | - | nd | - | 18 (n=228) | - | nd | - | |||||||
| SMN-N194 | 100 (n=56) | - | nd | - | 11 (n=107) | - | nd | - | |||||||
| SMN-Tudor | 100 (n=80) | - | 34 (n=300) | - | 12 (n=100) | - | nd | - | |||||||
| SMN472Δ5† | 100 (n=61) | 100 | 16 (n=108) | 16 | 13 (n=100) | 13 | 16 (n=189) | 16 | |||||||
| SMN472Δ5M2 | 80 (n=100) | - | nd | - | 16 (n=100) | - | nd | - | |||||||
| SMN-N86 | 100 (n=101) | - | 88 (n=172) | - | 53 (n=406) | - | nd | - | |||||||
| SMNE134K† | 76 (n=206) | 52 | nd | nd | 58 (n=202) | 54 | 41 (n=200) | 41 | |||||||
| . | Proportion of cells with . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | P80-CB (%) . | P80-positive FP foci (%) . | Endogenous SMN foci (%) . | Endogenous SMN-positive FP foci (%) . | TMG foci (%) . | TMG-positive FP foci (%) . | U2B” foci (%) . | U2B”-positive FP foci (%) . | |||||||
| Untransfected | 75 (n=717) | - | 71 (n=704) | - | 51 (n=205) | - | 39 (n=200) | - | |||||||
| Constructs* | |||||||||||||||
| SMN† | 100 (n=79) | 100 | nd | nd | 46 (n=100) | 46 | 32 (n=412) | 32 | |||||||
| SMNΔN86† | 95 (n=83) | 100 | 88 (n=100) | 88 | 9 (n=109) | 9 | 5 (n=221) | 5 | |||||||
| SMNΔex7† | 100 (n=132) | 100 | nd | - | 5 (n=183) | 5 | 6 (n=217) | 6 | |||||||
| SMNΔC40 | 80 (n=106) | - | nd | - | 18 (n=228) | - | nd | - | |||||||
| SMN-N194 | 100 (n=56) | - | nd | - | 11 (n=107) | - | nd | - | |||||||
| SMN-Tudor | 100 (n=80) | - | 34 (n=300) | - | 12 (n=100) | - | nd | - | |||||||
| SMN472Δ5† | 100 (n=61) | 100 | 16 (n=108) | 16 | 13 (n=100) | 13 | 16 (n=189) | 16 | |||||||
| SMN472Δ5M2 | 80 (n=100) | - | nd | - | 16 (n=100) | - | nd | - | |||||||
| SMN-N86 | 100 (n=101) | - | 88 (n=172) | - | 53 (n=406) | - | nd | - | |||||||
| SMNE134K† | 76 (n=206) | 52 | nd | nd | 58 (n=202) | 54 | 41 (n=200) | 41 | |||||||
*Presented in the same order as in Fig. 2A; †fusion proteins accumulated in CBs; nd, not determined.