Capacitive Ca2+ influx is increased in DD keratinocytes
| . | Control . | DD . |
|---|---|---|
| Cytosolic Ca2+ in EGTA (nM) | 31.44±12.15 (n=50) | 34.76±12.12 (n=17) |
| Thapsigargin peak (in EGTA) (nM) | 92.83±29.20 (n=48) | 41.82±16.51 (n=17)* |
| Peak capacitive Ca2+ (nM) | 108.55±60.41 (n=33) | 144.38±62.82 (n=17)* |
| . | Control . | DD . |
|---|---|---|
| Cytosolic Ca2+ in EGTA (nM) | 31.44±12.15 (n=50) | 34.76±12.12 (n=17) |
| Thapsigargin peak (in EGTA) (nM) | 92.83±29.20 (n=48) | 41.82±16.51 (n=17)* |
| Peak capacitive Ca2+ (nM) | 108.55±60.41 (n=33) | 144.38±62.82 (n=17)* |
Capacitive Ca2+ influx is larger in DD vs control keratinocytes, consistent with diminished ER Ca2+ stores. Note also that the response to thapsigargin is almost absent in DD keratinocytes, confirming that DD ER keratinocyte Ca2+ stores are severely depleted. n=number of cells. Data are presented as the mean ± s.d. *P<0.05 compared with control values, assessed using a two-tailed Student's t-test.