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Table 1.

S. cerevisiae strains used in this study

Strain Genotype Reference
CUY72  MATa pep4Δ::HIS3 prb1-Δ1.6R HIS3 lys2-208 trp1-Δ101 ura3-52 gal2 can vac8Δ::TRP1 Veit et al., 2001  
CUY73  MATa leu2-3 leu2-112 ura3-52 his3-Δ200 trp1-Δ101 lys2-801 suc2-Δ9 pho8:: TRP1 vac8Δ::HIS3 Veit et al., 2001  
CUY363a   CUY72; VAC8pr::pRS406-VAC8pr-VAC8  This study  
CUY363b   CUY73; VAC8pr::pRS406-VAC8pr-VAC8  This study  
CUY861a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys4  This study  
CUY861b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys4  This study  
CUY367a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys5  This study  
CUY367b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys5  This study  
CUY366a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys7  This study  
CUY366b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys7  This study  
CUY352a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys4,5  This study  
CUY352b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys4,5  This study  
CUY365a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys4,7  This study  
CUY365b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys4,7  This study  
CUY364a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys5,7  This study  
CUY364b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys5,7  This study  
CUY369a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys–  This study  
CUY369b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys–  This study  
CUY1092a   CUY72; VAC8pr::pRS406-VAC8pr-Src1-16-Vac8Δ1-18  This study  
CUY1092b   CUY73; VAC8pr::pRS406-VAC8pr-Src1-16-Vac8Δ1-18  This study  
CUY1615   CUY72; VAC8pr::pRS406-VAC8pr-Src1-16(G2A)-Vac8Δ1-18  This study  
CUY940   CUY72; pRS426- NOP1pr-VAC8-GFP  This study  
CUY973   CUY72; pRS426- NOP1pr-Vac8Cys4-GFP  This study  
CUY1042   CUY72; pRS426- NOP1pr-Vac8Cys5-GFP  This study  
CUY1063   CUY72; pRS426- NOP1pr-Vac8Cys7-GFP  This study  
CUY948   CUY72; pRS426- NOP1pr-Vac8Cys-GFP  This study  
CUY1549   CUY72; pRS426- NOP1pr-Vac8G2A-GFP  This study  
CUY987   CUY72; pRS426- NOP1pr-Src1-16-GFP  This study  
CUY942   CUY72; pRS426- NOP1pr-Vac81-18-GFP  This study  
CUY1006   CUY72; pRS426- NOP1pr-Src1-16-Vac8Δ1-18-GFP  This study  
CUY1229   CUY73; pRS426- NOP1pr-Vac81-18 Cys5-GFP  This study  
CUY1230   CUY73; pRS426- NOP1pr-Vac81-18 Cys7-GFP  This study  
CUY1909   CUY73; VAC8pr::pRS406-VAC8pr- Src1-16; S4C-Vac8Δ1-18  This study  
Strain Genotype Reference
CUY72  MATa pep4Δ::HIS3 prb1-Δ1.6R HIS3 lys2-208 trp1-Δ101 ura3-52 gal2 can vac8Δ::TRP1 Veit et al., 2001  
CUY73  MATa leu2-3 leu2-112 ura3-52 his3-Δ200 trp1-Δ101 lys2-801 suc2-Δ9 pho8:: TRP1 vac8Δ::HIS3 Veit et al., 2001  
CUY363a   CUY72; VAC8pr::pRS406-VAC8pr-VAC8  This study  
CUY363b   CUY73; VAC8pr::pRS406-VAC8pr-VAC8  This study  
CUY861a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys4  This study  
CUY861b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys4  This study  
CUY367a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys5  This study  
CUY367b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys5  This study  
CUY366a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys7  This study  
CUY366b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys7  This study  
CUY352a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys4,5  This study  
CUY352b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys4,5  This study  
CUY365a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys4,7  This study  
CUY365b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys4,7  This study  
CUY364a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys5,7  This study  
CUY364b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys5,7  This study  
CUY369a   CUY72; VAC8pr::pRS406-VAC8pr-Vac8Cys–  This study  
CUY369b   CUY73; VAC8pr::pRS406-VAC8pr-Vac8Cys–  This study  
CUY1092a   CUY72; VAC8pr::pRS406-VAC8pr-Src1-16-Vac8Δ1-18  This study  
CUY1092b   CUY73; VAC8pr::pRS406-VAC8pr-Src1-16-Vac8Δ1-18  This study  
CUY1615   CUY72; VAC8pr::pRS406-VAC8pr-Src1-16(G2A)-Vac8Δ1-18  This study  
CUY940   CUY72; pRS426- NOP1pr-VAC8-GFP  This study  
CUY973   CUY72; pRS426- NOP1pr-Vac8Cys4-GFP  This study  
CUY1042   CUY72; pRS426- NOP1pr-Vac8Cys5-GFP  This study  
CUY1063   CUY72; pRS426- NOP1pr-Vac8Cys7-GFP  This study  
CUY948   CUY72; pRS426- NOP1pr-Vac8Cys-GFP  This study  
CUY1549   CUY72; pRS426- NOP1pr-Vac8G2A-GFP  This study  
CUY987   CUY72; pRS426- NOP1pr-Src1-16-GFP  This study  
CUY942   CUY72; pRS426- NOP1pr-Vac81-18-GFP  This study  
CUY1006   CUY72; pRS426- NOP1pr-Src1-16-Vac8Δ1-18-GFP  This study  
CUY1229   CUY73; pRS426- NOP1pr-Vac81-18 Cys5-GFP  This study  
CUY1230   CUY73; pRS426- NOP1pr-Vac81-18 Cys7-GFP  This study  
CUY1909   CUY73; VAC8pr::pRS406-VAC8pr- Src1-16; S4C-Vac8Δ1-18  This study  
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