Effects of NOS inhibitor, NO and peroxynitrite (ONOO–) scavengers on neuronal survival following KA injection
| Treatment* . | Function . | % Survival† . |
|---|---|---|
| PBS | — | 26±16 |
| TMPyP (2 μM) | Nonfunctional | 20±5.3 |
| NMMA (400 μM) | Global NOS blocker | 84±7.4‡ |
| PTIO (300 μM) | NO scavenger | 93±2.9‡ |
| FeTMPyP (2 μM) | ONOO- and O2- scavenger | 97±4.3‡ |
| FeTPPS (10 μM) | Selective ONOO- scavenger | 88±16‡ |
| Treatment* . | Function . | % Survival† . |
|---|---|---|
| PBS | — | 26±16 |
| TMPyP (2 μM) | Nonfunctional | 20±5.3 |
| NMMA (400 μM) | Global NOS blocker | 84±7.4‡ |
| PTIO (300 μM) | NO scavenger | 93±2.9‡ |
| FeTMPyP (2 μM) | ONOO- and O2- scavenger | 97±4.3‡ |
| FeTPPS (10 μM) | Selective ONOO- scavenger | 88±16‡ |
Mice were treated with indicated compounds using an osmotic pump prior to, and following, KA injection.
Neural survival was determined by staining with Cresyl Blue and quantitation was performed with Scion Image beta 4.02. Statistical analysis was performed using a two-tailed t test.
P<0.05 compared with untreated mice. Error represents s.e.m. Each experimental group contained six mice.