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Table 2.

Inhibition of salt-induced actin polymerisation by thymosin β4 and its labelled derivatives assayed by falling ball viscometry

Sample Time of fall (seconds)
Buffer   10±2 (n=5)  
Actin   155±9 (n=5)  
Actin + thymosin β4  14±4 (n=5)  
Actin + thymosin β4(OGC)2  12±3 (n=5)  
Sample Time of fall (seconds)
Buffer   10±2 (n=5)  
Actin   155±9 (n=5)  
Actin + thymosin β4  14±4 (n=5)  
Actin + thymosin β4(OGC)2  12±3 (n=5)  

G-actin (4 μM) was incubated with equimolar amounts of the investigated peptides for 15 minutes at room temperature. Polymerisation was started by adding MgCl2 to a final concentration of 2 mM. Immediately after adding MgCl2 the mixture was filled into a glass capillary. After incubation for 4 hours at room temperature, viscosity was determined by falling ball viscometry.

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