Table 1.

Characterisation of fluorescently labelled peptides and fragments thereof by MALDI-TOF mass spectrometry

Peptide m/z (observed) Composition m/z (calculated) Deviation (‰)
Thymosin β4  4963.0   [Tβ4-H+]   4964.4   0.28  
Labelled peptide 1   5445.4   [Tβ4(OGC)1-H+]   5443.9   0.28  
Labelled peptide 2   5924.4   [Tβ4(OGC)2-H+]   5923.4   0.17  
    Fragment 1   2350.2   [Tβ427-43(OGC)1-H+]   2350.4   0.09  
    Fragment 2   3591.4   [Tβ41-26(OGC)1-H+]   3590.1   0.36  
Peptide m/z (observed) Composition m/z (calculated) Deviation (‰)
Thymosin β4  4963.0   [Tβ4-H+]   4964.4   0.28  
Labelled peptide 1   5445.4   [Tβ4(OGC)1-H+]   5443.9   0.28  
Labelled peptide 2   5924.4   [Tβ4(OGC)2-H+]   5923.4   0.17  
    Fragment 1   2350.2   [Tβ427-43(OGC)1-H+]   2350.4   0.09  
    Fragment 2   3591.4   [Tβ41-26(OGC)1-H+]   3590.1   0.36  

Peptides or fragments were isolated by preparative HPLC. Aliquots of the collected fractions were concentrated in vacuo and then applied to MALDI-TOF-MS.

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