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Table 1.

Effect of ConA and hypertonic sucrose on internalization of β1AR and β2AR and cholera toxin B-subunit in BHK cells

% Internalized
Treatment β1AR β2AR CT-B
None   24.9±3.5   60.6±2.1   34.6±6.2  
0.25 mg/ml Con A   4.85±1.9   14.8±4.75   39.1±5.4* 
0.6 M Sucrose   1.92±3.6   31.1±1.6   35.8±5.1* 
% Internalized
Treatment β1AR β2AR CT-B
None   24.9±3.5   60.6±2.1   34.6±6.2  
0.25 mg/ml Con A   4.85±1.9   14.8±4.75   39.1±5.4* 
0.6 M Sucrose   1.92±3.6   31.1±1.6   35.8±5.1* 

Cells expressing β1AR or β2AR were treated with the indicated inhibitor for 30 minutes, incubated in the absence and presence of 1 μM ISO for an additional 30 minutes and assayed for surface receptors as described in Materials and Methods. In addition, some of the cells were incubated with 10 nM CT-B for 30 minutes at 4°C, washed and either kept at 4°C or warmed in medium containing fresh inhibitor for 1 hour, and assayed for surface CT-B. Results shown represent the mean±s.e.m. of three to five independent experiments. *Not significant compared with no inhibitor.

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