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Table 1.

Plasmids used in this study

Plasmid Description Reference
pSC2   pGBT9-GEA2(aa 557-769)  This study  
pSC59-2   pASΔ-GEA1  This study  
pSC8   pRSET5d-GEA2(aa 557-769)  This study  
pSC9   pRSET5d-His6-HA-GEA2(aa 557-769)  This study  
pSC10   pRSET5d-DRS2(aa 1230-1355)  This study  
pSC11   pRSET5d-His6-HA-DRS2(aa 1230-1355)  This study  
pSC12   pRSET5d-DRS2(aa 1230-1305)  This study  
pSC13   pRSET5d-DRS2(aa 1305-1355)  This study  
pGMS3  2μ, URA3, GEA2  This study  
pGMS3-HA  2μ, URA3, GEA2-3x HA  This study  
pSKP1  CEN, URA3, GEA2  This study  
pRS315-DRS2  CEN, LEU2, DRS2  (Chen et al., 1999)  
pRS425-DRS2  2μ, LEU2, DRS2  (Chen et al., 1999)  
pCM116C  CEN, LEU2, TPIprom-KEX2-HA  (Mullins and Bonifacino, 2001)  
Plasmid Description Reference
pSC2   pGBT9-GEA2(aa 557-769)  This study  
pSC59-2   pASΔ-GEA1  This study  
pSC8   pRSET5d-GEA2(aa 557-769)  This study  
pSC9   pRSET5d-His6-HA-GEA2(aa 557-769)  This study  
pSC10   pRSET5d-DRS2(aa 1230-1355)  This study  
pSC11   pRSET5d-His6-HA-DRS2(aa 1230-1355)  This study  
pSC12   pRSET5d-DRS2(aa 1230-1305)  This study  
pSC13   pRSET5d-DRS2(aa 1305-1355)  This study  
pGMS3  2μ, URA3, GEA2  This study  
pGMS3-HA  2μ, URA3, GEA2-3x HA  This study  
pSKP1  CEN, URA3, GEA2  This study  
pRS315-DRS2  CEN, LEU2, DRS2  (Chen et al., 1999)  
pRS425-DRS2  2μ, LEU2, DRS2  (Chen et al., 1999)  
pCM116C  CEN, LEU2, TPIprom-KEX2-HA  (Mullins and Bonifacino, 2001)  
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