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Table 2.

Plasmids used in this study

Plasmid Relevant allele Marker Integration site Strains generated
pCDK-FLAG  cdk1-1  HygR cdk1 locus   TAU17  
pKOCDK  cdk1Δ::hph  HygR cdk1 locus   TAU4  
pCLB1-VSV  clb1-1  HygR clb1 locus   UMP19  
pKOCLB1  clb1Δ::hph  HygR clb1 locus   UMP21  
pCLB1nar  clb1nar  CbxR clb1 locus   TAU41, UMP25  
pRU11-CLB1   Pcrg1clb1-1  CbxR cbx1 locus   TAU36-1  
pRU12-CLB1   Pcrg1*clb1-1  CbxR cbx1 locus   TAU36-2  
pRU12-CLB1Δdb1   Pcrg1*clb1Δdb1  CbxR cbx1 locus   TAU30  
pRU12-CLB1Δdb2   Pcrg1*clb1Δdb2  CbxR cbx1 locus   TAU45  
pRU12-CLB1Δdb1-2   Pcrg1*clb1Δdb1-2  CbxR cbx1 locus   TAU31, TAU57  
pCLB2-MYC  clb2-1  HygR clb2 locus   UMP27  
pKOCLB2  clb2Δ::cbx  CbxR clb2 locus   UMP32  
pCLB2nar  clb2na  CbxR clb2 locus   TAU42, UMP26  
pRU11-CLB2   Pcrg1clb2-1  CbxR cbx1 locus   TAU52-1  
pRU12-CLB2   Pcrg1*clb2-1  CbxR cbx1 locus   TAU52-2  
pRU12-CLB2Δdb   Pcrg1*clb2Δdb  CbxR cbx1 locus   TAU53, TAU58  
pCU1-CLB2   Phsp70clb2-1  CbxR cbx1 locus   TAU26  
Plasmid Relevant allele Marker Integration site Strains generated
pCDK-FLAG  cdk1-1  HygR cdk1 locus   TAU17  
pKOCDK  cdk1Δ::hph  HygR cdk1 locus   TAU4  
pCLB1-VSV  clb1-1  HygR clb1 locus   UMP19  
pKOCLB1  clb1Δ::hph  HygR clb1 locus   UMP21  
pCLB1nar  clb1nar  CbxR clb1 locus   TAU41, UMP25  
pRU11-CLB1   Pcrg1clb1-1  CbxR cbx1 locus   TAU36-1  
pRU12-CLB1   Pcrg1*clb1-1  CbxR cbx1 locus   TAU36-2  
pRU12-CLB1Δdb1   Pcrg1*clb1Δdb1  CbxR cbx1 locus   TAU30  
pRU12-CLB1Δdb2   Pcrg1*clb1Δdb2  CbxR cbx1 locus   TAU45  
pRU12-CLB1Δdb1-2   Pcrg1*clb1Δdb1-2  CbxR cbx1 locus   TAU31, TAU57  
pCLB2-MYC  clb2-1  HygR clb2 locus   UMP27  
pKOCLB2  clb2Δ::cbx  CbxR clb2 locus   UMP32  
pCLB2nar  clb2na  CbxR clb2 locus   TAU42, UMP26  
pRU11-CLB2   Pcrg1clb2-1  CbxR cbx1 locus   TAU52-1  
pRU12-CLB2   Pcrg1*clb2-1  CbxR cbx1 locus   TAU52-2  
pRU12-CLB2Δdb   Pcrg1*clb2Δdb  CbxR cbx1 locus   TAU53, TAU58  
pCU1-CLB2   Phsp70clb2-1  CbxR cbx1 locus   TAU26  
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