PDE activities in REF52 cell lysates
| Inhibitor present in assay . | PDE activity in lysate (pmol/min/mg lysate protein) . | Inhibition (%) . |
|---|---|---|
| None | 11.5±0.1 | 0 |
| Cilostamide (1μM) | 8.9±0.8 | 23±7 |
| Rolipram (10μM) | 2.8±0.3 | 76±3 |
| Inhibitor present in assay . | PDE activity in lysate (pmol/min/mg lysate protein) . | Inhibition (%) . |
|---|---|---|
| None | 11.5±0.1 | 0 |
| Cilostamide (1μM) | 8.9±0.8 | 23±7 |
| Rolipram (10μM) | 2.8±0.3 | 76±3 |
REF52 cell lysates were prepared and assayed for PDE activity using 1 μM cAMP as substrate. Assays were carried out in the absence of any inhibitor, in the presence of the PDE3 selective inhibitor cilostamide (1 μM) or in the presence of the PDE4 selective inhibitor rolipram (10 μM). Activities are expressed as mean±s.e.m. and were determined in three independent assays.