The Company of Biologists

Table 1.

Localization patterns of AtMinD1-GFP fusion proteins in tobacco leaf cells^*

		Number of speckles per chloroplast (%)^{^‡}							Total number of chloroplasts (%)
Protein	Number of cells	0	1	2	3	4	>5		Total number of chloroplasts (%)
AtMinD1	40	58 (15.8)	223 (60.9)	47 (12.8)	13 (3.6)	9 (2.5)	16 (4.4)	336 (100)
AtMinD1 (A296G)	56	0 (0.0)	140 (31.9)	91 (20.7)	65 (14.8)	42 (9.6)	101 (23.0)	439 (100)

		Number of speckles per chloroplast (%)^{^‡}							Total number of chloroplasts (%)
Protein	Number of cells	0	1	2	3	4	>5		Total number of chloroplasts (%)
AtMinD1	40	58 (15.8)	223 (60.9)	47 (12.8)	13 (3.6)	9 (2.5)	16 (4.4)	336 (100)
AtMinD1 (A296G)	56	0 (0.0)	140 (31.9)	91 (20.7)	65 (14.8)	42 (9.6)	101 (23.0)	439 (100)

The experiment was performed by particle bombardment using sections from single leaf.

Fluorescence speckles were counted by Nikon TE2000 microscope and Hamamatsu ORCA-ER cooled CCD camera and OpenLab software system.