The nuclei of HeLa cells labelled for IFN-α1 mRNA, gag mRNA or luc-CTE mRNA, and CRM1 (shown in Fig. 4C,F,I) were analyzed using the Zeiss LSM 510 image examiner software (v. 3.2) as described in Materials and Methods. The colocalisation coefficients of each mRNAb and CRM1c as well as the Pearson's correlation coefficientsd were then measured using the software functions.