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Table 1.

PCR primer pairs

Amplified region Primer pair
IB   Forward: 5′-AACCCTAAGAACAACACGTTCACTAG-3′   Reverse: 5′-CTACAGGGCTCTGCCCTTCATTTTC-3′  
LIM-IB   Forward: 5′-ATGAATGTGCAGGCCTGCTCT-3′   Reverse: 5′-CTACAGGGCTCTGCCCTTCATTTTC-3′  
IB-SR   Forward: 5′-AACCCTAAGAACAACACGTTCACTAG-3′   Reverse: 5′-CTACACACAGGGAACACCACGCATG-3′  
LIM-SR    
   LIM segment   Forward: 5′-ATGAATGTGCAGGCCTGCTCT-3′   Reverse: 5′-TGGTTAAGGTCTAGAGTGGGCGTGACAGTACGG-3′  
   SR segment   Forward: 5′-ACACACACATCTAGAGGAGCCACAGACTCTAAGCTTCTGC-3′   Reverse: 5′-CTACACACAGGGAACACCACGCATG-3′  
Amplified region Primer pair
IB   Forward: 5′-AACCCTAAGAACAACACGTTCACTAG-3′   Reverse: 5′-CTACAGGGCTCTGCCCTTCATTTTC-3′  
LIM-IB   Forward: 5′-ATGAATGTGCAGGCCTGCTCT-3′   Reverse: 5′-CTACAGGGCTCTGCCCTTCATTTTC-3′  
IB-SR   Forward: 5′-AACCCTAAGAACAACACGTTCACTAG-3′   Reverse: 5′-CTACACACAGGGAACACCACGCATG-3′  
LIM-SR    
   LIM segment   Forward: 5′-ATGAATGTGCAGGCCTGCTCT-3′   Reverse: 5′-TGGTTAAGGTCTAGAGTGGGCGTGACAGTACGG-3′  
   SR segment   Forward: 5′-ACACACACATCTAGAGGAGCCACAGACTCTAAGCTTCTGC-3′   Reverse: 5′-CTACACACAGGGAACACCACGCATG-3′  

Stop codons added to each reverse primer are indicated in bold. Underlined residues mark XbaI endonuclease sites used to ligate the LIM and SR regions prior to cloning into the GFP-expression plasmid.

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