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Table 2.

Implantation of intact blastocysts, Tr vesicles, and ICMs in the uteri of pseudopregnant mice

Category No. transferred No. of recipients No. of horns with decidual swelling No. of decidual swellings
Intact blastocysts   70   10   10 (100%)   33  
Tr vesicles   30   5   5 (100%)   12  
ICMs   32   5   0   0  
Category No. transferred No. of recipients No. of horns with decidual swelling No. of decidual swellings
Intact blastocysts   70   10   10 (100%)   33  
Tr vesicles   30   5   5 (100%)   12  
ICMs   32   5   0   0  

Intact blastocysts, Tr vesicles or isolated ICMs were transferred into uterine lumens of day-4 pseudopregnant recipients as described in the Materials and sethods. Recipients were killed 48 hours after blastocyst transfer (equivalent to day 6 of normal pregnancy) and examined for implantation sites. Implantation sites were checked by an intravenous injection of 0.1 ml of 1% Chicago Blue B dye in saline. Uterine horns that did not exhibit any implantation sites were flushed with saline to recover blastocysts or ICMs. Mice without implantation sites were excluded from the study. The numbers in the parentheses indicates the percentage of recipients with implantation sites.

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