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Table 3.

Functionality assays

ConstructGrowth assayImport assayConstructGrowth assayImport assay
NuI-Pex1p − − Pex22-NuI (+) (+) 
NuI-Pex3p (+) (+) Pex2-Cub-RUra3p (+) (+) 
NuI-Pex4p (+) Pex3-Cub-RUra3p (+) 
NuI-Pex5p −  Pex4-Cub-RUra3p (+) (+) 
NuI-Pex10p (+) Pex5-Cub-RUra3p − − 
NuI-Pex11p Pex10-Cub-RUra3p (+) (+) 
NuI-Pex12p (+) −/(+) Pex11-Cub-RUra3p 
Pex12-NuIp − − Pex12-Cub-RUra3p − − 
NuI-Pex13p (+) (+) Pex13-Cub-RUra3p − − 
NuI-Pex14p − − Pex14-Cub-RUra3p − −/(+) 
NuI-Pex15p (+) Pex17-Cub-RUra3p (+) 
NuI-Pex19p Pex22-Cub-RUra3p 
ConstructGrowth assayImport assayConstructGrowth assayImport assay
NuI-Pex1p − − Pex22-NuI (+) (+) 
NuI-Pex3p (+) (+) Pex2-Cub-RUra3p (+) (+) 
NuI-Pex4p (+) Pex3-Cub-RUra3p (+) 
NuI-Pex5p −  Pex4-Cub-RUra3p (+) (+) 
NuI-Pex10p (+) Pex5-Cub-RUra3p − − 
NuI-Pex11p Pex10-Cub-RUra3p (+) (+) 
NuI-Pex12p (+) −/(+) Pex11-Cub-RUra3p 
Pex12-NuIp − − Pex12-Cub-RUra3p − − 
NuI-Pex13p (+) (+) Pex13-Cub-RUra3p − − 
NuI-Pex14p − − Pex14-Cub-RUra3p − −/(+) 
NuI-Pex15p (+) Pex17-Cub-RUra3p (+) 
NuI-Pex19p Pex22-Cub-RUra3p 

The results of the functionality assays of the Pex-fusion proteins by growth on oleic acid and GFP-SKL import are indicated. +, full functionality;(+), partial functionality; −, no functionality.

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