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Table 1.

Effect of chemical manipulation of vesicles on fiber assembly

Vesicle treatmentFiber assembly*
KPM assembly buffer Yes 
1.0 M KCI Yes 
0.7 M Na2CO3, pH 10 Yes 
Pronase (0.55 mg/ml) No 
Trypsin (0.55 mg/ml) No 
5 mM DTT/10 mM NEM No 
Vesicle treatmentFiber assembly*
KPM assembly buffer Yes 
1.0 M KCI Yes 
0.7 M Na2CO3, pH 10 Yes 
Pronase (0.55 mg/ml) No 
Trypsin (0.55 mg/ml) No 
5 mM DTT/10 mM NEM No 
*

Assayed by addition of vesicles treated as indicated to cytosol in the presence of 1 mM ATP.

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