Effect of chemical manipulation of vesicles on fiber assembly
| Vesicle treatment . | Fiber assembly* . |
|---|---|
| KPM assembly buffer | Yes |
| 1.0 M KCI | Yes |
| 0.7 M Na2CO3, pH 10 | Yes |
| Pronase (0.55 mg/ml) | No |
| Trypsin (0.55 mg/ml) | No |
| 5 mM DTT/10 mM NEM | No |
| Vesicle treatment . | Fiber assembly* . |
|---|---|
| KPM assembly buffer | Yes |
| 1.0 M KCI | Yes |
| 0.7 M Na2CO3, pH 10 | Yes |
| Pronase (0.55 mg/ml) | No |
| Trypsin (0.55 mg/ml) | No |
| 5 mM DTT/10 mM NEM | No |
Assayed by addition of vesicles treated as indicated to cytosol in the presence of 1 mM ATP.