S. pombe strains used in this study
| Strain . | Genotype . | Source . |
|---|---|---|
| HM123 | h- leu1-32 | Laboratory stock |
| 428h | h- ade6-M210 leu1-32 ura4-D18 | Laboratory stock |
| 429h | h+ ade6-M216 leu1-32 ura4-D18 | Laboratory stock |
| rad3Δ | h- rad3::ura4+ ade6-704 leu1-32 ura4-D18 | A. M. Carr |
| pds5Δ | h+ pds5::ura4+ ade6-M210 leu1-32 ura4-D18 | This study |
| h- pds5::ura4+ ade6-M216 leu1-32 ura4-D18 | This study | |
| pds5Δ (LEU2) | h- pds5::LEU2 ade6-M216 leu1-32 ura4-D18 | This study |
| HM248 | h- his2 ade6-M210 (Ch16 ade6-M216) | T. Toda |
| pds5Δ Ch16 | h- pds5::ura4+ ade6-M210 ura4-D18 (Ch16 ade6-M216) | This study |
| pds5-GFP | h- pds5-GFP:kanMX6 leu1-32 ura4-D18 | This study |
| pds5-HA | h- pds5-HA:kanMX6 leu1-32 ura4-D18 | This study |
| nda3-KM311 | h+ nda3-KM311 leu1-32 ura4-D18 his2 | T. Toda |
| nda3-KM311 pds5Δ | h+ nda3-KM311 pds5::ura4+ leu1-32 ura4-D18 | This study |
| ndc80-GFP | h- ndc80-GFP:kanMX6 leu1-32 ura4-D18 | J. V. Kilmartin |
| nda3-KM311 ndc80-GFP pds5Δ | h+ nda3-KM311 pds5::ura4 ndc80-GFP:kanMX6 leu1-32 ura4-D18 | This study |
| rad21-K1 | h- rad21-K1:ura4+ ade6-M216 leu1-32 ura4-D18 his7-366 | H. Ikeda |
| rad21-K1 pds5-GFP | h- rad21-K1:ura4+ pds5-GFP:kanMX6 ade6-M216 leu1-32 ura4-D18 his7-366 | This study |
| rad21-HA | h- rad21-HA:LEU2 leu1-32 | M. Yanagida |
| rad21-GFP | h- rad21-GFP:LEU2 leu1-32 | M. Yanagida |
| pds5Δ rad21-GFP | h- rad21-GFP:LEU2 pds5::ura4+ leu1-32 ura4-D18 | This study |
| rad21-K1 pds5Δ | h+ h- rad21-K1:ura4/rad21+pds5::LEU2/pds5+ ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 | This study |
| mis4-242 | h- mis4-242 leu1-32 | M. Yanagida |
| pds5Δ mis4-242 | h- mis4-242 pds5::LEU2 leu1-32 | This study |
| bub1Δ | h- bub1::LEU2 ade6-M210 leu1-32 ura4-DS/E his1-102 | J.-P. Javerzat |
| pds5Δ bub1Δ | h- pds5::ura4+ bub1::LEU2 ade6-M210 leu1-32 ura4-D18/DS/E | This study |
| GFP-swi6 | h- ars1(MluI)::pREP81Xgfpswi6-LEU2 ade6-M210 leu1-32 ura4-D18 | R. Allshire |
| GFP-swi6 pds5Δ | h- pds5::ura4+ ars1(MluI)::pREP81Xgfpswi6-LEU2 ade6-M210 leu1-32 ura4-D18 | This study |
| Strain . | Genotype . | Source . |
|---|---|---|
| HM123 | h- leu1-32 | Laboratory stock |
| 428h | h- ade6-M210 leu1-32 ura4-D18 | Laboratory stock |
| 429h | h+ ade6-M216 leu1-32 ura4-D18 | Laboratory stock |
| rad3Δ | h- rad3::ura4+ ade6-704 leu1-32 ura4-D18 | A. M. Carr |
| pds5Δ | h+ pds5::ura4+ ade6-M210 leu1-32 ura4-D18 | This study |
| h- pds5::ura4+ ade6-M216 leu1-32 ura4-D18 | This study | |
| pds5Δ (LEU2) | h- pds5::LEU2 ade6-M216 leu1-32 ura4-D18 | This study |
| HM248 | h- his2 ade6-M210 (Ch16 ade6-M216) | T. Toda |
| pds5Δ Ch16 | h- pds5::ura4+ ade6-M210 ura4-D18 (Ch16 ade6-M216) | This study |
| pds5-GFP | h- pds5-GFP:kanMX6 leu1-32 ura4-D18 | This study |
| pds5-HA | h- pds5-HA:kanMX6 leu1-32 ura4-D18 | This study |
| nda3-KM311 | h+ nda3-KM311 leu1-32 ura4-D18 his2 | T. Toda |
| nda3-KM311 pds5Δ | h+ nda3-KM311 pds5::ura4+ leu1-32 ura4-D18 | This study |
| ndc80-GFP | h- ndc80-GFP:kanMX6 leu1-32 ura4-D18 | J. V. Kilmartin |
| nda3-KM311 ndc80-GFP pds5Δ | h+ nda3-KM311 pds5::ura4 ndc80-GFP:kanMX6 leu1-32 ura4-D18 | This study |
| rad21-K1 | h- rad21-K1:ura4+ ade6-M216 leu1-32 ura4-D18 his7-366 | H. Ikeda |
| rad21-K1 pds5-GFP | h- rad21-K1:ura4+ pds5-GFP:kanMX6 ade6-M216 leu1-32 ura4-D18 his7-366 | This study |
| rad21-HA | h- rad21-HA:LEU2 leu1-32 | M. Yanagida |
| rad21-GFP | h- rad21-GFP:LEU2 leu1-32 | M. Yanagida |
| pds5Δ rad21-GFP | h- rad21-GFP:LEU2 pds5::ura4+ leu1-32 ura4-D18 | This study |
| rad21-K1 pds5Δ | h+ h- rad21-K1:ura4/rad21+pds5::LEU2/pds5+ ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 | This study |
| mis4-242 | h- mis4-242 leu1-32 | M. Yanagida |
| pds5Δ mis4-242 | h- mis4-242 pds5::LEU2 leu1-32 | This study |
| bub1Δ | h- bub1::LEU2 ade6-M210 leu1-32 ura4-DS/E his1-102 | J.-P. Javerzat |
| pds5Δ bub1Δ | h- pds5::ura4+ bub1::LEU2 ade6-M210 leu1-32 ura4-D18/DS/E | This study |
| GFP-swi6 | h- ars1(MluI)::pREP81Xgfpswi6-LEU2 ade6-M210 leu1-32 ura4-D18 | R. Allshire |
| GFP-swi6 pds5Δ | h- pds5::ura4+ ars1(MluI)::pREP81Xgfpswi6-LEU2 ade6-M210 leu1-32 ura4-D18 | This study |