ECM components produced by mesenchymal and epithelial cells used in this study
| . | LN1 (α1β1γ1) . | LN2 (α2β1γ1) . | LN5 (α3β3γ2) . | LN10 (α5β1γ1) . | Col IV . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| C9 | no α1 [1]a | α2 + [1]a | γ2 + [1]a | α5 ++ [2]a | + [1]c | |||||
| C11 | no α1 [1]a | α2 + [1]a | γ2 + [1]a | α5 ++ [2]a | + [1]c | |||||
| C20 | no α1 [1]a | α2 + [1]a | γ2 + [1]a | α5 ++ [2]a | + [1]c | |||||
| 129CB3* | α1 + [7,8]a | α2 + [7,8]c | α3 - [7]c | α5 + [7]c | + [6]c | |||||
| HT29MTX | no α1 RNAa | nd | α3 ++ [3]b,d | α5 + [2]a,d | nd | |||||
| no protein [2]d | β3 ++ [3]d | |||||||||
| γ2 ++ [3]b,d | ||||||||||
| Caco-2 | α1 RNA +a | α2 + [2]a | α3 - [3]b | α5 + [2]a,b | α5 + [9]c | |||||
| no protein [5]b | β3 - [3]d | [5]d | ||||||||
| γ2 + [3]b,d | ||||||||||
| The nature of ECM components produced by mesenchymal (C9; C11; C20) or epithelial cells from intestine (HT29-MTX; Caco-2) was determined by RT-PCR(a), Western blot (b), IF (c), or IP (d) by(Fritsch et al., 1999) [1],Simon-Assmann et al. (personal communication) [2],(Orian-Rousseau et al., 1998)[3], (De Arcangelis et al.,1996) [4], (Velling et al.,1999) [5]. *The composition of the native ECM secreted and deposited by mesenchymal muscle cells (129CB3) is deduced from studies on similar cell lines (G8; C2C12) from mouse skeletal muscle by(Rao et al., 1985) [6],(Patton et al., 1997) [7],(Vachon et al., 1996) [8](Beaulieu, 1997) [9]. nd=not determined. | ||||||||||
| . | LN1 (α1β1γ1) . | LN2 (α2β1γ1) . | LN5 (α3β3γ2) . | LN10 (α5β1γ1) . | Col IV . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| C9 | no α1 [1]a | α2 + [1]a | γ2 + [1]a | α5 ++ [2]a | + [1]c | |||||
| C11 | no α1 [1]a | α2 + [1]a | γ2 + [1]a | α5 ++ [2]a | + [1]c | |||||
| C20 | no α1 [1]a | α2 + [1]a | γ2 + [1]a | α5 ++ [2]a | + [1]c | |||||
| 129CB3* | α1 + [7,8]a | α2 + [7,8]c | α3 - [7]c | α5 + [7]c | + [6]c | |||||
| HT29MTX | no α1 RNAa | nd | α3 ++ [3]b,d | α5 + [2]a,d | nd | |||||
| no protein [2]d | β3 ++ [3]d | |||||||||
| γ2 ++ [3]b,d | ||||||||||
| Caco-2 | α1 RNA +a | α2 + [2]a | α3 - [3]b | α5 + [2]a,b | α5 + [9]c | |||||
| no protein [5]b | β3 - [3]d | [5]d | ||||||||
| γ2 + [3]b,d | ||||||||||
| The nature of ECM components produced by mesenchymal (C9; C11; C20) or epithelial cells from intestine (HT29-MTX; Caco-2) was determined by RT-PCR(a), Western blot (b), IF (c), or IP (d) by(Fritsch et al., 1999) [1],Simon-Assmann et al. (personal communication) [2],(Orian-Rousseau et al., 1998)[3], (De Arcangelis et al.,1996) [4], (Velling et al.,1999) [5]. *The composition of the native ECM secreted and deposited by mesenchymal muscle cells (129CB3) is deduced from studies on similar cell lines (G8; C2C12) from mouse skeletal muscle by(Rao et al., 1985) [6],(Patton et al., 1997) [7],(Vachon et al., 1996) [8](Beaulieu, 1997) [9]. nd=not determined. | ||||||||||