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Table 1.

Quantitation of NCS-1 labeling in neuronal perikarya: gold particles/μm2

Tubulovesicular elementsMitochondriaLysosomesNuclei
NCS-1 108±15.2 (0.77 μm2/7) 19±2.3 (1.82 μm2/6) 17±3.8 (1.2 μm2/5) 9±0.6 1.77 μm2/3) 
IgG 7±3.8 (0.91 μm2/8) 7±1.3 (3.92 μm2/13) 11±5.5 (0.38 μm2/6) 1 (0.77 μm2/1) 
Tubulovesicular elementsMitochondriaLysosomesNuclei
NCS-1 108±15.2 (0.77 μm2/7) 19±2.3 (1.82 μm2/6) 17±3.8 (1.2 μm2/5) 9±0.6 1.77 μm2/3) 
IgG 7±3.8 (0.91 μm2/8) 7±1.3 (3.92 μm2/13) 11±5.5 (0.38 μm2/6) 1 (0.77 μm2/1) 

Values given are means±s.e.m. The area (μm2) analyzed and the number of micrographs used for quantitation are shown in brackets. Results are from at least two separate experiments in which rat brain ultra thin sections were immunolabeled using anti-NCS-1 antibody or non-immune IgG and revealed by anti-rabbit IgG conjugated to 12 nm gold particles.

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