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Table 1.

Percentage amino acid identity of AmqKirA and AmqKirB with Nematostella vectensis (Nv) and vertebrate Kir channels calculated from global alignments with LALIGN online software(http://www.ch.embnet.org/software/LALIGN_form.html)for the overall channel sequence and the channel core (transmembrane regions M1 and M2 and pore region)

% Identity with AmqKirA
% Identity with AmqKirB
SequenceOverallCoreOverallCore
AmqKirA – – 67.8 69.2 
AmqKirB 67.8 69.2 – – 
Nv gi156375411 35.4 46.8 34.6 46.8 
Nv gi156372465 29.2 54.4 29.6 50.6 
Nv gi156395183 32.7 50.6 33.5 50.6 
Nv gi156387460 32.7 51.9 31.7 48.1 
rKir1.1 28.4 44.3 28.7 48.1 
mKir2.1 29.5 46.8 28.2 38.0 
hKir2.2 30.1 51.3 29.5 46.2 
hKir2.3 27.9 46.2 28.1 44.9 
hKir2.4 26.9 44.9 25.7 38.5 
hKir3.1 23.3 39.7 24.5 42.3 
hKir3.2 30.2 47.4 28.9 43.6 
hKir3.3 29.1 47.4 27.8 41.0 
hKir3.4 29.3 44.9 28.6 44.9 
hKir4.1 25.9 38.0 27.1 41.8 
hKir4.2 25.3 42.3 26.6 42.3 
hKir5.1 24.8 41.0 24.3 37.2 
hKir6.1 25.1 43.0 26.6 44.3 
hKir6.2 28.7 49.4 28.6 43.0 
hKir7.1 25.0 43.6 23.2 39.7 
% Identity with AmqKirA
% Identity with AmqKirB
SequenceOverallCoreOverallCore
AmqKirA – – 67.8 69.2 
AmqKirB 67.8 69.2 – – 
Nv gi156375411 35.4 46.8 34.6 46.8 
Nv gi156372465 29.2 54.4 29.6 50.6 
Nv gi156395183 32.7 50.6 33.5 50.6 
Nv gi156387460 32.7 51.9 31.7 48.1 
rKir1.1 28.4 44.3 28.7 48.1 
mKir2.1 29.5 46.8 28.2 38.0 
hKir2.2 30.1 51.3 29.5 46.2 
hKir2.3 27.9 46.2 28.1 44.9 
hKir2.4 26.9 44.9 25.7 38.5 
hKir3.1 23.3 39.7 24.5 42.3 
hKir3.2 30.2 47.4 28.9 43.6 
hKir3.3 29.1 47.4 27.8 41.0 
hKir3.4 29.3 44.9 28.6 44.9 
hKir4.1 25.9 38.0 27.1 41.8 
hKir4.2 25.3 42.3 26.6 42.3 
hKir5.1 24.8 41.0 24.3 37.2 
hKir6.1 25.1 43.0 26.6 44.3 
hKir6.2 28.7 49.4 28.6 43.0 
hKir7.1 25.0 43.6 23.2 39.7 

Vertebrate Kir sequences are given for human (hKirx.x), rat (rKir1.1) and mouse (mKir2.1) channels, which were used as controls in our electrophysiology experiments

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