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Table 1.

The primers and probes used

GeneSequence
shh Forward: 5′-GGCGTTTGCACCTGTCAGA-3′ 
 Reverse: 5′-TGGGCATGGAGTATTCTTTCG-3′ 
 Probe: 6-FAM-TACTGGCTGTCCTCGTTGCGA-TAMRA 
col I Forward: 5′-GAGGGTGGATGCAGGTGTGT-3′ 
 Reverse: 5′-TACTGGATCGACCCCAACCA-3′ 
 Probe: 6-FAM-TGGAGAAGTCGCAGTGGGCCTTGAT-TAMRA 
alp Forward: 5′-CTACACGCCAAGAGGGAACAC-3′ 
 Reverse: 5′-GGTAAAGGGTTTCTGGTCCACAT-3′ 
 Probe: 6-FAM-ATTTTTGGACTGGCTCCCATGTTGAGT-TAMRA 
mgp Forward: 5′-GAAAGCACAGAATCCTTTGAAGATGT-3′ 
 Reverse: 5′-GTGGACTCTGTGGGTTGATGAA-3′ 
 Probe: 6-FAM-TTTGTCAGTCCATACCGAGCCAAC-TAMRA 
e1α Forward: 5′-CCCCTCCAGGACGTTTACAAA-3′ 
 Reverse: 5′-CACACGGCCCACAGGTACA-3′ 
 Probe: 6-FAM-ATCGGTGGTATTGGA-MGB 
GeneSequence
shh Forward: 5′-GGCGTTTGCACCTGTCAGA-3′ 
 Reverse: 5′-TGGGCATGGAGTATTCTTTCG-3′ 
 Probe: 6-FAM-TACTGGCTGTCCTCGTTGCGA-TAMRA 
col I Forward: 5′-GAGGGTGGATGCAGGTGTGT-3′ 
 Reverse: 5′-TACTGGATCGACCCCAACCA-3′ 
 Probe: 6-FAM-TGGAGAAGTCGCAGTGGGCCTTGAT-TAMRA 
alp Forward: 5′-CTACACGCCAAGAGGGAACAC-3′ 
 Reverse: 5′-GGTAAAGGGTTTCTGGTCCACAT-3′ 
 Probe: 6-FAM-ATTTTTGGACTGGCTCCCATGTTGAGT-TAMRA 
mgp Forward: 5′-GAAAGCACAGAATCCTTTGAAGATGT-3′ 
 Reverse: 5′-GTGGACTCTGTGGGTTGATGAA-3′ 
 Probe: 6-FAM-TTTGTCAGTCCATACCGAGCCAAC-TAMRA 
e1α Forward: 5′-CCCCTCCAGGACGTTTACAAA-3′ 
 Reverse: 5′-CACACGGCCCACAGGTACA-3′ 
 Probe: 6-FAM-ATCGGTGGTATTGGA-MGB 
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