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Table 3.

Quantification of IsoAspartate (IsoAsp), deamidation and the IsoAsp:Aspartate (IsoAspartate:aspartate) ratio in aged recombinant thread matrix proteins (rTMP)

rTMP treatmentIsoAsp (mol/mol ±s.d.)Deamidation (mean/peptide)IsoAsp:Asp (approximate)
Non-aged Da,b 1.4 (4.2)c – 
37°C 3 days 1.22±0.10b 4.9 (14.7)c 1:3 
37°C 6 days 1.81±0.09b 5.7 (17.1)c 1:2 
14°C 6 days 0.19±0.03b 2.4 (7.2)c 1:12 
rTMP treatmentIsoAsp (mol/mol ±s.d.)Deamidation (mean/peptide)IsoAsp:Asp (approximate)
Non-aged Da,b 1.4 (4.2)c – 
37°C 3 days 1.22±0.10b 4.9 (14.7)c 1:3 
37°C 6 days 1.81±0.09b 5.7 (17.1)c 1:2 
14°C 6 days 0.19±0.03b 2.4 (7.2)c 1:12 
a

None detected

b

N=3

c

Number in parentheses is the estimated number of deamidation events for a full-length TMP

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