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Table 2.

TG accumulation, and ACC and FAS activity in goose primary hepatocytes after treatment with insulin or glucose

Insulin (nmoll^–1)	Glucose (mmoll^–1)	TG accumulation (mmoll^–1)	FAS activity^{^*}	ACC activity^{^†}
0	0	0.725±0.064^c	169.0±7.211^d	105.14±9.98^c
50	–	0.890±0.014^c	181.3±18.148^d	107.53±10.49^c
100	–	1.145±0.035^b	195.3±20.648^c	126.53±11.52^b
150	–	1.320±0.028^a	223.3±21.385^b	132.76±11.31^b
200	–	0.340±0.113^d	121.3±5.508^e	86.61±8.23^d
–	5	0.855±0.050^c	189.7±11.590^d	106.61±10.25^c
–	30	1.675±0.007^a	213.7±15.044^b	110.28±10.31^c
50	5	1.125±0.261^b	231.7±15.503^b	121.53±10.28^b
50	30	1.485±0.431^a	256.3±14.572^a	156.12±11.18^a

Insulin (nmoll^–1)	Glucose (mmoll^–1)	TG accumulation (mmoll^–1)	FAS activity^{^*}	ACC activity^{^†}
0	0	0.725±0.064^c	169.0±7.211^d	105.14±9.98^c
50	–	0.890±0.014^c	181.3±18.148^d	107.53±10.49^c
100	–	1.145±0.035^b	195.3±20.648^c	126.53±11.52^b
150	–	1.320±0.028^a	223.3±21.385^b	132.76±11.31^b
200	–	0.340±0.113^d	121.3±5.508^e	86.61±8.23^d
–	5	0.855±0.050^c	189.7±11.590^d	106.61±10.25^c
–	30	1.675±0.007^a	213.7±15.044^b	110.28±10.31^c
50	5	1.125±0.261^b	231.7±15.503^b	121.53±10.28^b
50	30	1.485±0.431^a	256.3±14.572^a	156.12±11.18^a

TG, triglyceride

Different lowercase letters in the same array indicate a significant difference between treatments (P<0.05). After 24 h in serum-free medium, hepatocytes were incubated for 48 h either with no addition as a control or with 50 nmoll^–1 insulin, 100 nmoll^–1 insulin, 150 nmoll^–1 insulin, 200 nmoll^–1 insulin, 5 mmoll^–1 glucose, 30 mmoll^–1 glucose, 50 nmoll^–1 insulin + 5 mmoll^–1 glucose or 50 nmoll^–1 insulin + 30 mmoll^–1 glucose added. The standard culture medium contained 25 mmoll^–1 glucose before additions

Activity given as nmoles of substrate (NADPH) transformed to NADP per min per mg of cytosolic protein

†

nmoles of substrate (H[¹⁴C]O₃^–)fixed to malonyl CoA per min per g of cytosolic protein

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