PCR primers
| Gene . | Upstream primer (5′–3′) . | Downstream primer (5′–3′) . | Product size (bp) . |
|---|---|---|---|
| SREBP-1 | CGAGTACATCCGCTTCCTGC | TGAGGGACTTGCTCTTCTGC | 92 |
| FAS | TGGGAGTAACACTGATGGC | TCCAGGCTTGATACCACA | 109 |
| ACCα | TGCCTCCGAGAACCCTAA | AAGACCACTGCCACTCCA | 163 |
| Gene . | Upstream primer (5′–3′) . | Downstream primer (5′–3′) . | Product size (bp) . |
|---|---|---|---|
| SREBP-1 | CGAGTACATCCGCTTCCTGC | TGAGGGACTTGCTCTTCTGC | 92 |
| FAS | TGGGAGTAACACTGATGGC | TCCAGGCTTGATACCACA | 109 |
| ACCα | TGCCTCCGAGAACCCTAA | AAGACCACTGCCACTCCA | 163 |
SREBP-1, sterol regulatory element-binding protein-1; ACCα, acetyl CoA carboxylase-α; and FAS, fatty acid synthase