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Table 1.

Description of experimental manipulations of the flow and odor environment for P. americana males walking upwind in a pheromone plume in a laboratory wind tunnel (Series 2 experiment)

Experimental treatmentStimulus exposure prior to releaseExplanation
Continuous odor plume in wind Odor and Wind Placed in center of experimental arena. Continuous exposure to wind and odor throughout performance. 
Pre-exposure to odor and wind Odor and Wind Placed in center of experimental arena. Wind stopped just prior to release into stationary odor plume. 
Pre-exposure to wind only Wind Placed in arena, outside of odor plume to ensure no exposure to odor. Wind stopped just prior to release into stationary odor plume. 
No exposure to wind or odor None Placed in a sealed container in arena outside of plume to ensure no exposure to wind or odor. Wind stopped just prior to release into stationary odor plume. 
Experimental treatmentStimulus exposure prior to releaseExplanation
Continuous odor plume in wind Odor and Wind Placed in center of experimental arena. Continuous exposure to wind and odor throughout performance. 
Pre-exposure to odor and wind Odor and Wind Placed in center of experimental arena. Wind stopped just prior to release into stationary odor plume. 
Pre-exposure to wind only Wind Placed in arena, outside of odor plume to ensure no exposure to odor. Wind stopped just prior to release into stationary odor plume. 
No exposure to wind or odor None Placed in a sealed container in arena outside of plume to ensure no exposure to wind or odor. Wind stopped just prior to release into stationary odor plume. 
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