Sequences of the forward and reverse primers used in this study and the length of the expected PCR amplicon
| Gene amplified . | GenBank accession no. . | Forward primer . | Reverse primer . | Product (bp) . |
|---|---|---|---|---|
| MyoD I | AJ557148 | 5′GTGCTTGGATAAAAACGTGCGC3′ | 5′CAAAGAAATGCATGTCGC3′ | 195 |
| MyoD II | AJ557149 | 5′GAGCCAGGATTACACTCGTTAC3′ | 5′CGAAGAAATGCATGTCGC3′ | 141 |
| Myogenin | AJ534875 | 5′GTGGAGATCCTGAGGAGTGC3′ | 5′GGTCCTCGTTGCTGTAGCTC3′ | 232 |
| β-Actin | AF012125 | 5′CGCCGCACTGGTTGTTGACA3′ | 5′GCGGTGCCCATCTCCTGCT3′ | 674 |
| 18S | AJ427629 | 5′GGAGGTTCGAAGACGATCAG3′ | 5′CTCAATCTCGTGTGGCTGAA3′ | 438 |
| GH transgene | (Dietch et al., 2006) | 5′GCTCTTCAACATCGCGGTCA3′ | 5′ATATGGAGCAGCTTCAGGGAC3′ | 207 |
| Gene amplified . | GenBank accession no. . | Forward primer . | Reverse primer . | Product (bp) . |
|---|---|---|---|---|
| MyoD I | AJ557148 | 5′GTGCTTGGATAAAAACGTGCGC3′ | 5′CAAAGAAATGCATGTCGC3′ | 195 |
| MyoD II | AJ557149 | 5′GAGCCAGGATTACACTCGTTAC3′ | 5′CGAAGAAATGCATGTCGC3′ | 141 |
| Myogenin | AJ534875 | 5′GTGGAGATCCTGAGGAGTGC3′ | 5′GGTCCTCGTTGCTGTAGCTC3′ | 232 |
| β-Actin | AF012125 | 5′CGCCGCACTGGTTGTTGACA3′ | 5′GCGGTGCCCATCTCCTGCT3′ | 674 |
| 18S | AJ427629 | 5′GGAGGTTCGAAGACGATCAG3′ | 5′CTCAATCTCGTGTGGCTGAA3′ | 438 |
| GH transgene | (Dietch et al., 2006) | 5′GCTCTTCAACATCGCGGTCA3′ | 5′ATATGGAGCAGCTTCAGGGAC3′ | 207 |
GH, growth hormone