The Company of Biologists

Skip to Main Content

Table 1.

HPLC gradient program for carbohydrate derivative separation

Time (min)	Solvent A (%)	Solvent B (%)
0 (initial conditions)	90	10
5.5	90	10
6	80	20
12	66	34
13 (wash)	0	100
16	0	100
18	90	10
23	90	10

Time (min)	Solvent A (%)	Solvent B (%)
0 (initial conditions)	90	10
5.5	90	10
6	80	20
12	66	34
13 (wash)	0	100
16	0	100
18	90	10
23	90	10

Changes in solvent composition over time are linear. See Materials and methods for details of solvent composition