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Table 4.

Body composition of ad-libitum-fed mice and mice working for food

Independent t-test
Variable (g)Ad-lib animals (N=7)Workload animals (N=15)Difference (%)tP
Body mass 31.4±0.9 25.9±0.4 -17 -6.8 <0.001 
Dl mass 8.1±0.2 6.6±0.1 -19 -9.9 <0.001 
Fat content 3.13±0.35 0.94±0.09 -70 -8.2 <0.001 
Dl heart 0.04±0.001 0.03±0.001 -29 -4.8 <0.001 
Dl liver 0.48±0.03 0.30±0.02 -37 -5.0 <0.001 
Dl kidney 0.13±0.01 0.10±0.001 -29 -4.0 0.001 
Dl brain 0.08±0.001 0.08±0.001 -3 -1.3 0.23 
Dl stomach 0.04±0.001 0.04±0.001 +5 0.9 0.36 
Dl intestines 0.29±0.01 0.34±0.01 +18 4.5 <0.001 
Dl lung 0.04±0.001 0.04±0.001 +3 0.6 0.59 
Dl skin 1.50±0.04 1.26±0.02 -16 -6.0 <0.001 
Dl rest 5.48±0.12 4.35±0.05 -21 -10.6 <0.001 
Fat heart 0.006±0.001 0.005±0.001 -18 -1.1 0.30 
Fat liver 0.048±0.009 0.027±0.003 -43 -2.8 0.011 
Fat kidney 0.026±0.003 0.006±0.001 -75 -7.7 <0.001 
Fat brain 0.047±0.001 0.043±0.001 -10 -3.7 0.002 
Fat stomach 0.008±0.001 0.005±0.001 -36 -4.2 <0.001 
Fat intestines 0.070±0.010 0.039±0.002 -44 -4.2 <0.001 
Fat lung 0.008±0.001 0.004±0.001 -51 -6.7 <0.001 
Fat skin 0.85±0.120 0.16±0.028 -81 -7.6 <0.001 
Fat rest 2.07±0.240 0.65±0.071 -69 -7.4 <0.001 
Independent t-test
Variable (g)Ad-lib animals (N=7)Workload animals (N=15)Difference (%)tP
Body mass 31.4±0.9 25.9±0.4 -17 -6.8 <0.001 
Dl mass 8.1±0.2 6.6±0.1 -19 -9.9 <0.001 
Fat content 3.13±0.35 0.94±0.09 -70 -8.2 <0.001 
Dl heart 0.04±0.001 0.03±0.001 -29 -4.8 <0.001 
Dl liver 0.48±0.03 0.30±0.02 -37 -5.0 <0.001 
Dl kidney 0.13±0.01 0.10±0.001 -29 -4.0 0.001 
Dl brain 0.08±0.001 0.08±0.001 -3 -1.3 0.23 
Dl stomach 0.04±0.001 0.04±0.001 +5 0.9 0.36 
Dl intestines 0.29±0.01 0.34±0.01 +18 4.5 <0.001 
Dl lung 0.04±0.001 0.04±0.001 +3 0.6 0.59 
Dl skin 1.50±0.04 1.26±0.02 -16 -6.0 <0.001 
Dl rest 5.48±0.12 4.35±0.05 -21 -10.6 <0.001 
Fat heart 0.006±0.001 0.005±0.001 -18 -1.1 0.30 
Fat liver 0.048±0.009 0.027±0.003 -43 -2.8 0.011 
Fat kidney 0.026±0.003 0.006±0.001 -75 -7.7 <0.001 
Fat brain 0.047±0.001 0.043±0.001 -10 -3.7 0.002 
Fat stomach 0.008±0.001 0.005±0.001 -36 -4.2 <0.001 
Fat intestines 0.070±0.010 0.039±0.002 -44 -4.2 <0.001 
Fat lung 0.008±0.001 0.004±0.001 -51 -6.7 <0.001 
Fat skin 0.85±0.120 0.16±0.028 -81 -7.6 <0.001 
Fat rest 2.07±0.240 0.65±0.071 -69 -7.4 <0.001 

Values are mean ± s.e.m. Total dry lean (dl) mass, fat content and the dl mass and fat mass of separate organs are shown for Workload and Ad-lib mice. The body mass shown is the total body mass minus the gut and intestine content at time of death. One mouse died during the second respirometry measurement in the Workload group. Difference (%) shows the change in mass between Ad-lib and Workload animals. Independent t-tests were performed to test for differences between groups and the results are shown in the Table

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