The Company of Biologists

Table 2.

Primers used for real-time PCR and distribution analysis

Name	Orientation	Nucleotide sequence (5′ to 3′)
L8 F2	Sense	CGT TTC AAG AAA AGG ACG GAG C
L8 R2	Antisense	GGA GAT GAC GGT GGC GTA G
COX2 F2	Sense	ATC CTT ATT GGC GAG ACT ATC A
COX2 R2	Antisense	ATT GAG GGA TTG GTA ACG CAT TT
nNOS F3	Sense	ACC GTG TTT TGT CCG AAG TG
nNOS R3	Antisense	AAG GTG CTT GGT TGT CTT TG
NKA F2	Sense	AGC CCT GGT GGT ACG TGA TG
NKA R2	Antisense	GGA AGC TAA GGT GGC AAT ACG AC
NKCC F1	Sense	CCC GCA GCC ACT GGT ATT
NKCC R1	Antisense	GCC ATC TGT GGG TCA GCA A
CFTR F2	Sense	GGC GTC ATA CTG CGT AGT GTT AC
CFTR R2	Antisense	TCA TGC CTT CCT CTA AGC GTA G
COX2 DF1	Sense	TCA CTG AAG CCT ACG CCT AAC AC
COX2 DR1	Antisense	CTC CAG GTT CTC GCC ATA AA

Name	Orientation	Nucleotide sequence (5′ to 3′)
L8 F2	Sense	CGT TTC AAG AAA AGG ACG GAG C
L8 R2	Antisense	GGA GAT GAC GGT GGC GTA G
COX2 F2	Sense	ATC CTT ATT GGC GAG ACT ATC A
COX2 R2	Antisense	ATT GAG GGA TTG GTA ACG CAT TT
nNOS F3	Sense	ACC GTG TTT TGT CCG AAG TG
nNOS R3	Antisense	AAG GTG CTT GGT TGT CTT TG
NKA F2	Sense	AGC CCT GGT GGT ACG TGA TG
NKA R2	Antisense	GGA AGC TAA GGT GGC AAT ACG AC
NKCC F1	Sense	CCC GCA GCC ACT GGT ATT
NKCC R1	Antisense	GCC ATC TGT GGG TCA GCA A
CFTR F2	Sense	GGC GTC ATA CTG CGT AGT GTT AC
CFTR R2	Antisense	TCA TGC CTT CCT CTA AGC GTA G
COX2 DF1	Sense	TCA CTG AAG CCT ACG CCT AAC AC
COX2 DR1	Antisense	CTC CAG GTT CTC GCC ATA AA