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Table 1.

Parameters obtained from stretch and ATP step experiments at different pH

pH 6.7, controlpH 7.2pH 7.4pH 7.7
Stretch experiments     
t2 (ms) 454±60 (17) 639±89 (4) 803±84 (6) 1059±104 (7) 
t3 (ms) 1127±105 (17) 1596±126 (4) 1756±117 (6) 2040±131 (7) 
ATP step experiments     
ΔTfast decay (%) 44.8±7.2 (5) 65.5±8.3 (7) 
rslow decay (% min−12.0±0.1 (5) 2.2±0.1 (5) 
τfast decay (ms) 495±90 (5) 478±70 (7) 
t½fast decay (ms) 331±42 (5) 319±37 (7) 
τforce rise (ms) 590±43 (7) 1339±502 (7) 
t½force rise (ms) 441±48 (7) 1024±125 (7) 
pH 6.7, controlpH 7.2pH 7.4pH 7.7
Stretch experiments     
t2 (ms) 454±60 (17) 639±89 (4) 803±84 (6) 1059±104 (7) 
t3 (ms) 1127±105 (17) 1596±126 (4) 1756±117 (6) 2040±131 (7) 
ATP step experiments     
ΔTfast decay (%) 44.8±7.2 (5) 65.5±8.3 (7) 
rslow decay (% min−12.0±0.1 (5) 2.2±0.1 (5) 
τfast decay (ms) 495±90 (5) 478±70 (7) 
t½fast decay (ms) 331±42 (5) 319±37 (7) 
τforce rise (ms) 590±43 (7) 1339±502 (7) 
t½force rise (ms) 441±48 (7) 1024±125 (7) 

Values are means ± s.d. (N).

Parameters obtained from stretch experiments: t2, time from the beginning of stretch to the onset of delayed force increase; t3, time from the beginning of stretch to the peak of delayed force increase.

Parameters obtained from ATP step experiments: ΔTfast decay, extent of force decay within the initial rapid phase; rslow decay, the rate of force decay per min within the slow (almost linear) phase; τfast decay, t½fast decay, time constant and half-time, respectively, of force decay in the initial rapid phase;τ force rise, t½force rise, time constant and halftime, respectively, of force rise.

Significances for statistic differences: t2 and t3, P<0.01 for each test condition against control condition (paired Student-t-tests); τforce rise, t½force rise andΔ Tfast decay, P<0.01 for test and control condition (unpaired Student's t-tests); rslow decay, P<0.05 for test and control condition (unpaired Student's t-tests). The time parameters τfast decayand t½fast decay are not significantly different between test and control condition (P>0.23, unpaired Student's t-test).

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