Kinetic parameters of Na,K-ATPase in hearts from rats during activation with increasing concentrations of ATP
[ATP] activation
.
Vmax (μmol Pi mg-1 protein h)
.
Km (mmol ATP 1-1)
.
Control SL
2.71±0.12
0.487±0.058
SL+H.b.
3.05±0.20
0.353±0.048*
[ATP] activation
.
Vmax (μmol Pi mg-1 protein h)
.
Km (mmol ATP 1-1)
.
Control SL
2.71±0.12
0.487±0.058
SL+H.b.
3.05±0.20
0.353±0.048*
Six replicate experiments were performed for control sarcolemma alone(control SL), and sarcolemma with 3 μg of SGE-proteins from Hybomitra bimaculata (SL+H.b.).
Values are means ± s.e.m. *P<0.05 as compared to control group.