Effects of repeated pre- and post-branchial occlusions and sham-injections in rainbow trout Oncorhynchus mykiss
. | Untreated . | . | . | . | Sham I . | . | . | . | Sham II . | . | . | . | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | N . | Control . | Post . | Pre . | N . | Control . | Post . | Pre . | N . | Control . | Post . | Pre . | |||||||||
fh (beats min-1) | 9–10 | 58.2±3.5 | 43.5±3.7* | 80.7±4.1* | 7–9 | 61.8±3.7† | 47.9±4.4* | 76.2±4.2* | 7–9 | 66.5±3.3† | 55.8±3.8* | 79.2±4.7* | |||||||||
MCFP (kPa) | 9 | 0.12±0.03 | 0.13±0.04 | 0.23±0.04* | 8 | 0.19±0.04† | 0.20±0.04 | 0.29±0.04* | 7–8 | 0.20±0.03† | 0.23±0.04 | 0.31±0.05* |
. | Untreated . | . | . | . | Sham I . | . | . | . | Sham II . | . | . | . | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | N . | Control . | Post . | Pre . | N . | Control . | Post . | Pre . | N . | Control . | Post . | Pre . | |||||||||
fh (beats min-1) | 9–10 | 58.2±3.5 | 43.5±3.7* | 80.7±4.1* | 7–9 | 61.8±3.7† | 47.9±4.4* | 76.2±4.2* | 7–9 | 66.5±3.3† | 55.8±3.8* | 79.2±4.7* | |||||||||
MCFP (kPa) | 9 | 0.12±0.03 | 0.13±0.04 | 0.23±0.04* | 8 | 0.19±0.04† | 0.20±0.04 | 0.29±0.04* | 7–8 | 0.20±0.03† | 0.23±0.04 | 0.31±0.05* |
Values are means ± s.e.m. of heart rate(fh) and mean circulatory filling pressure (MCFP) in unstimulated control, post- and pre-branchial occlusion.
A sham-injection of physiological saline (1 ml kg-1), following the same experimental protocol as in Fig. 2, was administered.
Statistically significant difference from the control (P≤0.05)within each treatment
statistically significant difference between control values(P≤0.05)