Skip to Main Content
Table 2.

Relative contribution of rapid or instantaneous Cu accumulation in/on cells at time zero compared to net Cu accumulation over 15 min exposure to 800μmol l-1 Cu

Copper accumulation rate (nmol mg-1 cell protein h-1)
TreatmentTime 0 controlAfter 15 minInstantaneous Cu accumulation (%)
Normal Na+ 0.24±0.14a 1.88±0.52*,a 12.8 
Low Na+ 0.51±0.05b 3.04±0.69*,b 16.8 
Ice 4.63±0.89c,d 9.53±4.78c 48.5 
Amiloride(2 mmol l-13.00±0.38c,d 21.76±5.15*,d 13.8 
CDPC (10μmol l-16.39±2.01c 39.42±16.56*,d,g,e 16.2 
Phenamil(100 μmol l-16.33±1.36c 75.39±13.47*,e 8.4 
Low Cl- 2.58±0.47d 21.56±2.44*,d 12.0 
DIDS (100 μmol l-12.79±0.33d 18.40±0.94*,f 15.1 
pH 5.5 8.84±2.41e 32.65±3.70*,g 27.1 
Copper accumulation rate (nmol mg-1 cell protein h-1)
TreatmentTime 0 controlAfter 15 minInstantaneous Cu accumulation (%)
Normal Na+ 0.24±0.14a 1.88±0.52*,a 12.8 
Low Na+ 0.51±0.05b 3.04±0.69*,b 16.8 
Ice 4.63±0.89c,d 9.53±4.78c 48.5 
Amiloride(2 mmol l-13.00±0.38c,d 21.76±5.15*,d 13.8 
CDPC (10μmol l-16.39±2.01c 39.42±16.56*,d,g,e 16.2 
Phenamil(100 μmol l-16.33±1.36c 75.39±13.47*,e 8.4 
Low Cl- 2.58±0.47d 21.56±2.44*,d 12.0 
DIDS (100 μmol l-12.79±0.33d 18.40±0.94*,f 15.1 
pH 5.5 8.84±2.41e 32.65±3.70*,g 27.1 

Values are means ± s.e.m,N=5–6 experiments.

Time 0 control; cells were pipetted into saline containing 800 μmol l-1 Cu and then immediately washed in 0.1 μmol l-1EDTA solution.

After 15 min, Cu accumulation rate of cells at the end of a 15 min exposure to saline containing 800 μmol l-1 Cu.

% instantaneous Cu accumulation=time 0 control/after 15 min ×100%

*

Significantly different from time 0 control (within rows) by Student's t-test or Mann–Whitney U-test (P<0.05);values within columns with one or more common superscript letters are not significantly different from each other (Kruskal–Wallis test, P>0.05)

Cells were pre-incubated with the appropriate epithelial Na+channel inhibitor for 15 min prior to the addition of Cu

Close Modal

or Create an Account

Close Modal
Close Modal