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Table 1.

LDH release and stability of Na+ and K+ contents in freshly isolated trout intestinal cells

Time (min)
Treatment01530
Control (no added Cu)    
   Rate of LDH release <0.1 <0.1 <0.1 
      (μmol min-1 ml-1saline)    
   Cell [Na+11.8±1.0 12.3±2.3 13.0±4.8 
      (μmol mg-1 cell protein)    
   Cell [K+1.0±0.1 2.0±0.3 1.1±0.2 
      (μmol mg-1 cell protein)    
Copper (800 μmol l-1   
   Rate of LDH release <0.1 <0.1 <0.1 
      (μmol min-1 ml-1saline)    
   Cell [Na+15.4±2.9 14.6±1.3 15.6±1.8 
      (μmol mg-1 cell protein)    
   Cell [K+1.5±0.1 1.2±0.3 0.8±0.1 
      (μmol mg-1 cell protein)    
Time (min)
Treatment01530
Control (no added Cu)    
   Rate of LDH release <0.1 <0.1 <0.1 
      (μmol min-1 ml-1saline)    
   Cell [Na+11.8±1.0 12.3±2.3 13.0±4.8 
      (μmol mg-1 cell protein)    
   Cell [K+1.0±0.1 2.0±0.3 1.1±0.2 
      (μmol mg-1 cell protein)    
Copper (800 μmol l-1   
   Rate of LDH release <0.1 <0.1 <0.1 
      (μmol min-1 ml-1saline)    
   Cell [Na+15.4±2.9 14.6±1.3 15.6±1.8 
      (μmol mg-1 cell protein)    
   Cell [K+1.5±0.1 1.2±0.3 0.8±0.1 
      (μmol mg-1 cell protein)    

Values are means ± s.e.m., N=6 cell isolations.

Cells were incubated in physiological saline for 30 min immediately after the cell isolation protocol.

Control: cells in physiological saline without added Cu.

Copper: cells from the same isolations, but incubated in saline containing the highest Cu concentration (800 μmol l-1 Cu) used in experiments.

Rate of lactate dehydrogenase (LDH) release from cells to the medium remained below detection limit (<0.1 μmol ml-1 saline min-1) throughout in all treatments.

No statistically significant effects of time (within rows) were observed(Kruskal–Wallis test, P>0.05) in any of the electrolyte data. No treatment effects were observed at any time point (Student's t-test, P>0.05) for cell Na+ or K+.

Cells remain viable for at least 4 h (see text).

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