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Table 2.

Kinetic constants for transmural 65Zn2+ transport in the presence and absence of 20 μmol l–1l-histidine and 50 μmol l–1 copper

PerfusateKm (μmol l—1)Jmax (pmol cm—2 min—1)
Zinc control 267±37 0.07±0.003 
Zinc + l-Histidine 310±107 0.13±0.02* 
Zinc + l-Histidine + Cu+ 119±21 0.05±0.002 
Zinc + l-Histidine + Cu2+ 2079±1231 0.08±0.03 
PerfusateKm (μmol l—1)Jmax (pmol cm—2 min—1)
Zinc control 267±37 0.07±0.003 
Zinc + l-Histidine 310±107 0.13±0.02* 
Zinc + l-Histidine + Cu+ 119±21 0.05±0.002 
Zinc + l-Histidine + Cu2+ 2079±1231 0.08±0.03 

Values are means ± s.e.m. of three replicates per point.

*

Significantly different (P<0.01) than control

significantly different (P<0.01) than Zn +l-Histidine treatment

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