Conditions used to measure pH inside the midgut of adult mosquitoes using pH indicators and membrane feeding
| Treatment . | Media mixture* . | 0.02% indicator . | 0.001% DMSO . | 10-4 mol 1-1 Methazolamide . | 10-4 mol 1-1 Acetazolamide . |
|---|---|---|---|---|---|
| Control medium | + | + | - | - | - |
| Control + DMSO | + | + | + | - | - |
| Metazolamide + DMSO | + | + | + | + | + |
| Metazolamide + acetazolamide + DMSO | + | + | + | + | + |
| Treatment . | Media mixture* . | 0.02% indicator . | 0.001% DMSO . | 10-4 mol 1-1 Methazolamide . | 10-4 mol 1-1 Acetazolamide . |
|---|---|---|---|---|---|
| Control medium | + | + | - | - | - |
| Control + DMSO | + | + | + | - | - |
| Metazolamide + DMSO | + | + | + | + | + |
| Metazolamide + acetazolamide + DMSO | + | + | + | + | + |
For further explanation, see text.