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Table 1.

Chorioallantoic membrane (CAM) thickness (D_CAM),surface area (S_CAM) (calculated), blood vessel numerical density [N_A(v)] in the CAM, and area fraction of blood vessels [A_A(v)] under the air cell and under the rest of the eggshell of the chick embryo

	Embryonic age (day)
CAM sample zone	10	12	14	16	18	20	ANOVA (among days)
D_CAM (μm)
Air cell	60.6±22.8	64.9±13.2	35.1±15.7	69.6±28.7	69.6±22.0	89.1±25.3	P<0.001^*
Rest	67.4±26.4	79.8±26.1	49.4±20.0	77.0±35.9	85.7±21.0	110±38.3	P<0.001^*
t-test	N.S.	P<0.01	P<0.01	N.S.	N.S.	P<0.01
Air cell/rest ratio	1.0±0.3 (11)	0.8±0.2 (13)	0.7±0.3 (14)	1.0±0.5 (13)	0.9±0.3 (14)	0.8±0.2 (9)	N.S.^**
S_CAM (cm)
Air cell S_ac	9.6±0.4	10.9±0.4	12.5±0.5	13.9±0.8	15.0±0.5	16.0±0.8	P<0.001^*
Rest S_re	72.0±3.1	68.4±2.3	67.9±2.7	66.1±3.6	64.0±2.3	57.7±3.0	P<00.01^*
t-test	P<0.0001	P<0.0001	P<0.0001	P<0.0001	P<0.0001	P<0.0001
Air cell/rest ratio	0.13±0.06 (10)	0.16±0.05 (13)	0.18±0.06 (13)	0.21±0.08 (12)	0.23±0.06 (13)	0.25±0.07 (6)
N_A(v) (vessels mm^-2)
Air cell	102±19	127±25	141±23	164±49	153±24	122±27	P<0.001^*
Rest	62±10	84±11	88±11	93±39	83±13	61±17	P<0.001^*
t-test	P<0.001	P<0.001	P<0.002	P<0.001	P<0.001	P<0.001
Air cell/rest ratio	1.7±0.4 (10)	1.5±0.4 (13)	1.6±0.4 (13)	2.0±0.8 (12)	1.9±0.4 (13)	2.3±1.1 (6)	N.S.^**
A_A(v) (% of total area)
Air cell	33.5±11.0	32.2±10.5	40.0±10.1	38.1±7.6	36.3±9.8	42.1±14.4	N.S^*
Rest	24.0±10.8	18.8±6.1	21.8±4.8	17.6±4.1	18.5±3.9	21.6±7.2	N.S^*
t-test	P<0.03	P<<0.01	P<<0.01	P<<0.01	P<<0.01	P<0.01
Air cell/rest ratio	1.5±0.7 (11)	1.8±0.6 (13)	1.9±0.5 (14)	2.3±0.5 (13)	2.0±0.6 (14)	2.8±1.6 (9)	P<0.05^**

	Embryonic age (day)
CAM sample zone	10	12	14	16	18	20	ANOVA (among days)
D_CAM (μm)
Air cell	60.6±22.8	64.9±13.2	35.1±15.7	69.6±28.7	69.6±22.0	89.1±25.3	P<0.001^*
Rest	67.4±26.4	79.8±26.1	49.4±20.0	77.0±35.9	85.7±21.0	110±38.3	P<0.001^*
t-test	N.S.	P<0.01	P<0.01	N.S.	N.S.	P<0.01
Air cell/rest ratio	1.0±0.3 (11)	0.8±0.2 (13)	0.7±0.3 (14)	1.0±0.5 (13)	0.9±0.3 (14)	0.8±0.2 (9)	N.S.^**
S_CAM (cm)
Air cell S_ac	9.6±0.4	10.9±0.4	12.5±0.5	13.9±0.8	15.0±0.5	16.0±0.8	P<0.001^*
Rest S_re	72.0±3.1	68.4±2.3	67.9±2.7	66.1±3.6	64.0±2.3	57.7±3.0	P<00.01^*
t-test	P<0.0001	P<0.0001	P<0.0001	P<0.0001	P<0.0001	P<0.0001
Air cell/rest ratio	0.13±0.06 (10)	0.16±0.05 (13)	0.18±0.06 (13)	0.21±0.08 (12)	0.23±0.06 (13)	0.25±0.07 (6)
N_A(v) (vessels mm^-2)
Air cell	102±19	127±25	141±23	164±49	153±24	122±27	P<0.001^*
Rest	62±10	84±11	88±11	93±39	83±13	61±17	P<0.001^*
t-test	P<0.001	P<0.001	P<0.002	P<0.001	P<0.001	P<0.001
Air cell/rest ratio	1.7±0.4 (10)	1.5±0.4 (13)	1.6±0.4 (13)	2.0±0.8 (12)	1.9±0.4 (13)	2.3±1.1 (6)	N.S.^**
A_A(v) (% of total area)
Air cell	33.5±11.0	32.2±10.5	40.0±10.1	38.1±7.6	36.3±9.8	42.1±14.4	N.S^*
Rest	24.0±10.8	18.8±6.1	21.8±4.8	17.6±4.1	18.5±3.9	21.6±7.2	N.S^*
t-test	P<0.03	P<<0.01	P<<0.01	P<<0.01	P<<0.01	P<0.01
Air cell/rest ratio	1.5±0.7 (11)	1.8±0.6 (13)	1.9±0.5 (14)	2.3±0.5 (13)	2.0±0.6 (14)	2.8±1.6 (9)	P<0.05^**

Values are means ± s.d. (N). Student's t-tests (paired) are between zones for each day. ^*One-way ANOVA is among days. ^**Kruskal–Wallis ANOVA, median test is among days for the ratios under the air cell to the rest of the CAM.

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