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Table 1.

Chorioallantoic membrane (CAM) thickness (DCAM),surface area (SCAM) (calculated), blood vessel numerical density [NA(v)] in the CAM, and area fraction of blood vessels [AA(v)] under the air cell and under the rest of the eggshell of the chick embryo

Embryonic age (day)
CAM sample zone101214161820ANOVA (among days)
DCAM (μm)        
   Air cell 60.6±22.8 64.9±13.2 35.1±15.7 69.6±28.7 69.6±22.0 89.1±25.3 P<0.001* 
   Rest 67.4±26.4 79.8±26.1 49.4±20.0 77.0±35.9 85.7±21.0 110±38.3 P<0.001* 
   t-test N.S. P<0.01 P<0.01 N.S. N.S. P<0.01  
   Air cell/rest ratio 1.0±0.3 (11) 0.8±0.2 (13) 0.7±0.3 (14) 1.0±0.5 (13) 0.9±0.3 (14) 0.8±0.2 (9) N.S.** 
SCAM (cm)        
   Air cell Sac 9.6±0.4 10.9±0.4 12.5±0.5 13.9±0.8 15.0±0.5 16.0±0.8 P<0.001* 
   Rest Sre 72.0±3.1 68.4±2.3 67.9±2.7 66.1±3.6 64.0±2.3 57.7±3.0 P<00.01* 
   t-test P<0.0001 P<0.0001 P<0.0001 P<0.0001 P<0.0001 P<0.0001  
   Air cell/rest ratio 0.13±0.06 (10) 0.16±0.05 (13) 0.18±0.06 (13) 0.21±0.08 (12) 0.23±0.06 (13) 0.25±0.07 (6)  
NA(v) (vessels mm-2       
   Air cell 102±19 127±25 141±23 164±49 153±24 122±27 P<0.001* 
   Rest 62±10 84±11 88±11 93±39 83±13 61±17 P<0.001* 
   t-test P<0.001 P<0.001 P<0.002 P<0.001 P<0.001 P<0.001  
   Air cell/rest ratio 1.7±0.4 (10) 1.5±0.4 (13) 1.6±0.4 (13) 2.0±0.8 (12) 1.9±0.4 (13) 2.3±1.1 (6) N.S.** 
AA(v) (% of total area)        
   Air cell 33.5±11.0 32.2±10.5 40.0±10.1 38.1±7.6 36.3±9.8 42.1±14.4 N.S* 
   Rest 24.0±10.8 18.8±6.1 21.8±4.8 17.6±4.1 18.5±3.9 21.6±7.2 N.S* 
   t-test P<0.03 P<<0.01 P<<0.01 P<<0.01 P<<0.01 P<0.01  
   Air cell/rest ratio 1.5±0.7 (11) 1.8±0.6 (13) 1.9±0.5 (14) 2.3±0.5 (13) 2.0±0.6 (14) 2.8±1.6 (9) P<0.05** 
Embryonic age (day)
CAM sample zone101214161820ANOVA (among days)
DCAM (μm)        
   Air cell 60.6±22.8 64.9±13.2 35.1±15.7 69.6±28.7 69.6±22.0 89.1±25.3 P<0.001* 
   Rest 67.4±26.4 79.8±26.1 49.4±20.0 77.0±35.9 85.7±21.0 110±38.3 P<0.001* 
   t-test N.S. P<0.01 P<0.01 N.S. N.S. P<0.01  
   Air cell/rest ratio 1.0±0.3 (11) 0.8±0.2 (13) 0.7±0.3 (14) 1.0±0.5 (13) 0.9±0.3 (14) 0.8±0.2 (9) N.S.** 
SCAM (cm)        
   Air cell Sac 9.6±0.4 10.9±0.4 12.5±0.5 13.9±0.8 15.0±0.5 16.0±0.8 P<0.001* 
   Rest Sre 72.0±3.1 68.4±2.3 67.9±2.7 66.1±3.6 64.0±2.3 57.7±3.0 P<00.01* 
   t-test P<0.0001 P<0.0001 P<0.0001 P<0.0001 P<0.0001 P<0.0001  
   Air cell/rest ratio 0.13±0.06 (10) 0.16±0.05 (13) 0.18±0.06 (13) 0.21±0.08 (12) 0.23±0.06 (13) 0.25±0.07 (6)  
NA(v) (vessels mm-2       
   Air cell 102±19 127±25 141±23 164±49 153±24 122±27 P<0.001* 
   Rest 62±10 84±11 88±11 93±39 83±13 61±17 P<0.001* 
   t-test P<0.001 P<0.001 P<0.002 P<0.001 P<0.001 P<0.001  
   Air cell/rest ratio 1.7±0.4 (10) 1.5±0.4 (13) 1.6±0.4 (13) 2.0±0.8 (12) 1.9±0.4 (13) 2.3±1.1 (6) N.S.** 
AA(v) (% of total area)        
   Air cell 33.5±11.0 32.2±10.5 40.0±10.1 38.1±7.6 36.3±9.8 42.1±14.4 N.S* 
   Rest 24.0±10.8 18.8±6.1 21.8±4.8 17.6±4.1 18.5±3.9 21.6±7.2 N.S* 
   t-test P<0.03 P<<0.01 P<<0.01 P<<0.01 P<<0.01 P<0.01  
   Air cell/rest ratio 1.5±0.7 (11) 1.8±0.6 (13) 1.9±0.5 (14) 2.3±0.5 (13) 2.0±0.6 (14) 2.8±1.6 (9) P<0.05** 

Values are means ± s.d. (N). Student's t-tests (paired) are between zones for each day. *One-way ANOVA is among days. **Kruskal–Wallis ANOVA, median test is among days for the ratios under the air cell to the rest of the CAM.

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