Embryos used for quantitative RT-PCR at various developmental stages
| Hours post fertilization (h.p.f.) . | Stage . | Remarks . |
|---|---|---|
| 25 | 20 | 4 somite stage |
| 39 | 24 | 16 somite stage (start of heat beating) |
| 46 | 25 | 18-19 somite stage |
| 58 | 28 | 30 somite stage |
| 63 | 30 | 35 somite stage (blood vessel development) |
| 77 | 32 | Somite completion stage |
| 98 | 35 | Visceral blood vessels formation stage |
| 110 | 36 | Heart development stage |
| 122 | 37 | Pericardial cavity formation stage |
| 146 | 38 | Spleen development stage |
| 156 | 39 | Hatching stage |
| Hours post fertilization (h.p.f.) . | Stage . | Remarks . |
|---|---|---|
| 25 | 20 | 4 somite stage |
| 39 | 24 | 16 somite stage (start of heat beating) |
| 46 | 25 | 18-19 somite stage |
| 58 | 28 | 30 somite stage |
| 63 | 30 | 35 somite stage (blood vessel development) |
| 77 | 32 | Somite completion stage |
| 98 | 35 | Visceral blood vessels formation stage |
| 110 | 36 | Heart development stage |
| 122 | 37 | Pericardial cavity formation stage |
| 146 | 38 | Spleen development stage |
| 156 | 39 | Hatching stage |
Embryos were grown at 27°C.
Data are taken from Iwamatsu(1994).