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Table 1.

Embryos used for quantitative RT-PCR at various developmental stages

Hours post fertilization (h.p.f.)StageRemarks
25 20 4 somite stage 
39 24 16 somite stage (start of heat beating) 
46 25 18-19 somite stage 
58 28 30 somite stage 
63 30 35 somite stage (blood vessel development) 
77 32 Somite completion stage 
98 35 Visceral blood vessels formation stage 
110 36 Heart development stage 
122 37 Pericardial cavity formation stage 
146 38 Spleen development stage 
156 39 Hatching stage 
Hours post fertilization (h.p.f.)StageRemarks
25 20 4 somite stage 
39 24 16 somite stage (start of heat beating) 
46 25 18-19 somite stage 
58 28 30 somite stage 
63 30 35 somite stage (blood vessel development) 
77 32 Somite completion stage 
98 35 Visceral blood vessels formation stage 
110 36 Heart development stage 
122 37 Pericardial cavity formation stage 
146 38 Spleen development stage 
156 39 Hatching stage 

Embryos were grown at 27°C.

Data are taken from Iwamatsu(1994).

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