Table 1.

PCR primer sequences (5′→3′)

SequenceForwardReverse
S1MHC* CGAAACCAATGCTCTGAAGAACACC GTAAGCATCAAGTACCGTCCAAGG 
S2 MHC CGCCTCGAAGTTGAGGGTAAT ACAGGTGTTACTTGTCGGTGTCGG 
S1 Tm S2 Tm forward primer AAATAGGTTACAGGCCAGCTGAGG 
S2 Tm TCGACAGGCTCGAAGATGAACTTG AGCTTCGATAACGCTCACTACCAC 
SequenceForwardReverse
S1MHC* CGAAACCAATGCTCTGAAGAACACC GTAAGCATCAAGTACCGTCCAAGG 
S2 MHC CGCCTCGAAGTTGAGGGTAAT ACAGGTGTTACTTGTCGGTGTCGG 
S1 Tm S2 Tm forward primer AAATAGGTTACAGGCCAGCTGAGG 
S2 Tm TCGACAGGCTCGAAGATGAACTTG AGCTTCGATAACGCTCACTACCAC 

MHC, myosin heavy chain; Tm, tropomyosin; S1, slow-twitch muscle; S2, slow-tonic muscle.

*

Primers for S1 MHC are the same as used by Medler and Mykles(2003)

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.
Accept