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Table 1.

Preincubation and incubation procedure used during the first*experiment on inhibition of Pi uptake

Incubation components (mmol l-1 Ringer)
TreatmentPre-incubation*Incubation
Normal 5 Pi tracer Pi 
Na— choline 5 Pi, choline chloride tracer Pi, choline chloride 
Na— KCl 5 Pi, KCl tracer Pi, KCl 
Ouabain 5 Pi, 5 ouabain tracer Pi, 5 ouabain 
P++ 5 Pi tracer Pi, 10 Pi 
PFA 5 Pi, 5 PFA tracer Pi, 5 PFA 
NaN3 5 Pi, 10 NaN3 tracer Pi, 10 NaN3 
Cold 5 Pi tracer Pi, ice-cold 
Incubation components (mmol l-1 Ringer)
TreatmentPre-incubation*Incubation
Normal 5 Pi tracer Pi 
Na— choline 5 Pi, choline chloride tracer Pi, choline chloride 
Na— KCl 5 Pi, KCl tracer Pi, KCl 
Ouabain 5 Pi, 5 ouabain tracer Pi, 5 ouabain 
P++ 5 Pi tracer Pi, 10 Pi 
PFA 5 Pi, 5 PFA tracer Pi, 5 PFA 
NaN3 5 Pi, 10 NaN3 tracer Pi, 10 NaN3 
Cold 5 Pi tracer Pi, ice-cold 

Pre-incubation conditions were ice-cold (2°C), 30 min; incubation conditions were 15°C, 6 min.

Normal incubation medium was Ringer solution containing Pi as indicated.

Na— choline: iso-osmotic choline chloride replaced all NaCl (see text for details).

Na— KCl: iso-osmotic KCl replaced all NaCl (see text for details).

Tracer Pi, see text.

PFA, phosphonoformic acid; NaN3, sodium azide.

*

In experiment 2, tissues were pre-incubated at 15°C for 5 min in medium containing no Pi.

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