Number of water (-Δnw) and solute molecules(-Δns) released during transition from deoxy- to oxy-hemoglobin samples
. | -Δnw . | . | -Δns . | . | ||
---|---|---|---|---|---|---|
. | No CO2 . | +CO2 . | No CO2 . | +CO2 . | ||
HbI+II | 13.55±2.46 | 35.51±7.22 | -0.10±0.02 | -0.27±0.48 | ||
HbII+III | 3.54±2.70 | 7.96±5.04 | -0.02±0.02 | -0.05±0.04 | ||
Hemolysate | 10.33±0.03 | 16.81±3.76 | -0.09±0.01 | -0.15±0.03 | ||
RBC | - | 7.31±1.07 | - | -0.11±0.01 |
. | -Δnw . | . | -Δns . | . | ||
---|---|---|---|---|---|---|
. | No CO2 . | +CO2 . | No CO2 . | +CO2 . | ||
HbI+II | 13.55±2.46 | 35.51±7.22 | -0.10±0.02 | -0.27±0.48 | ||
HbII+III | 3.54±2.70 | 7.96±5.04 | -0.02±0.02 | -0.05±0.04 | ||
Hemolysate | 10.33±0.03 | 16.81±3.76 | -0.09±0.01 | -0.15±0.03 | ||
RBC | - | 7.31±1.07 | - | -0.11±0.01 |
Values are means ± S.E.M. calculated from the slopes of the plots in Figs 1, 2 and 4 according to Equations 2 and 3 in Materials and methods.
HbI+II, HbII+III and hemolysate were studied in presence and absence of 4%CO2, and red blood cells (RBC) in the presence of 0.5%CO2.
Values for RBC refer to the osmolality range 0.25-2.0 osmol kg-1.