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Table 1.

Intra-and extracellular acid-base variables of isolated hepatocytes prepared from P. brachycephalum

pHepHiPCO2 (kPa)[HCO3]i (mmol l−1)[HCO3]e (mmol l−1)
7.90 7.65±0.08 1.02 10.45 48.95 
7.90 7.58±0.05 0.03 0.39 1.54 
7.20 7.23±0.05 1.02 4.12 8.68 
7.20 7.17±0.07 0.03 0.15 0.27 
6.50 6.98±0.05 1.02 2.61 1.68 
6.50 6.98±0.07 0.03 0.08 0.05 
pHepHiPCO2 (kPa)[HCO3]i (mmol l−1)[HCO3]e (mmol l−1)
7.90 7.65±0.08 1.02 10.45 48.95 
7.90 7.58±0.05 0.03 0.39 1.54 
7.20 7.23±0.05 1.02 4.12 8.68 
7.20 7.17±0.07 0.03 0.15 0.27 
6.50 6.98±0.05 1.02 2.61 1.68 
6.50 6.98±0.07 0.03 0.08 0.05 

pHi values are means ± s.d., N=5-6.

Intracellular CO2 partial pressure was assumed to equal medium PCO2.

Apparent mean intracellular bicarbonate concentrations were calculated from mean pHi values by use of the Henderson-Hasselbach equation (see Materials and methods).

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