Characteristics of some insect chitinases
| . | MsCHT-1 . | BmCHT-1 . | AaCHT-1 . |
|---|---|---|---|
| Organism | Manduca | Bombyx | Aedes |
| sexta | mori | aegypti | |
| Accession no. | A56596 | AAB47538 | AAB81849 |
| Molecular mass (kDa) | |||
| Deduced from cDNA | 62.20 | 63.39 | 64.27 |
| Zymogenic forms* | 119 | 215 | |
| Active forms | 50, 62, 75, 97 | 54, 65, 88 | 33, 40 |
| pI | 5.32 | 5.15 | 4.83 |
| PEST sites | 404—437 | 417—440 | 394—408 |
| 474—508 | 471—499 | 413—436 | |
| 451—471 | |||
| Total number of N-glycosylation sites | 3 | 3 | 2 |
| Total number of O-glycosylation sites | 24 | 23 | 25 |
| . | MsCHT-1 . | BmCHT-1 . | AaCHT-1 . |
|---|---|---|---|
| Organism | Manduca | Bombyx | Aedes |
| sexta | mori | aegypti | |
| Accession no. | A56596 | AAB47538 | AAB81849 |
| Molecular mass (kDa) | |||
| Deduced from cDNA | 62.20 | 63.39 | 64.27 |
| Zymogenic forms* | 119 | 215 | |
| Active forms | 50, 62, 75, 97 | 54, 65, 88 | 33, 40 |
| pI | 5.32 | 5.15 | 4.83 |
| PEST sites | 404—437 | 417—440 | 394—408 |
| 474—508 | 471—499 | 413—436 | |
| 451—471 | |||
| Total number of N-glycosylation sites | 3 | 3 | 2 |
| Total number of O-glycosylation sites | 24 | 23 | 25 |
Prediction of putative PEST sites and N- and O-glycosylation sites was performed with the programs PESTfind,PROSCAN and NetOGlyc 2.0, respectively(Rogers et al., 1986; Bairoch et al., 1997; Hansen et al., 1997).
pI, isoelectric point.
Putative zymogenic forms were suggested based on their immunoreactivity with anti-chitinase antibodies after SDS-PAGE and western blots (Koga et al., 1989, 1992)