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Table 1.

Characteristics of some insect chitin synthases

	MsCHS-1	LcCHS-1	AaCHS-1	DmCHS-1, DmeChSB, kkv	DmCHS-2, DmeChSA	AgCHS-2	AgCHS-1
Organism	Manduca sexta	Lucilia cuprina	Aedes aegypti	Drosophila melanogaster	Drosophila melanogaster	Anopheles gambiae	Anopheles gambiae
Accession no.	AY062175	AF221067	AF223577	NM_079509	NM_079485	XM_321337	AY056833^{^*}
Class	A	A	B	A	B	A	B
Molecular mass (kDa)	178.56	180.72	179.39	182.83	161.39	186.40	181.27
pI	6.56	6.39	6.13	6.37	6.66	6.24	6.37
TMH	16	17	14	17	16	14	16
N-terminus	Outside	Inside	Outside	Inside	Outside	Outside	Outside
PEST sites	9—33	7—39	1147—1179	7—39	n.p.	7—30	n.p.
	1178—1205
Coiled-coils	1060—1094	1065—1099	n.p.	1078—1108	n.p.	1066—1098	n.p.
N-glycosylation sites
Total number	9	5	6	7	3	11	7
Conserved position	905	910	895	921	912	909	891

	MsCHS-1	LcCHS-1	AaCHS-1	DmCHS-1, DmeChSB, kkv	DmCHS-2, DmeChSA	AgCHS-2	AgCHS-1
Organism	Manduca sexta	Lucilia cuprina	Aedes aegypti	Drosophila melanogaster	Drosophila melanogaster	Anopheles gambiae	Anopheles gambiae
Accession no.	AY062175	AF221067	AF223577	NM_079509	NM_079485	XM_321337	AY056833^{^*}
Class	A	A	B	A	B	A	B
Molecular mass (kDa)	178.56	180.72	179.39	182.83	161.39	186.40	181.27
pI	6.56	6.39	6.13	6.37	6.66	6.24	6.37
TMH	16	17	14	17	16	14	16
N-terminus	Outside	Inside	Outside	Inside	Outside	Outside	Outside
PEST sites	9—33	7—39	1147—1179	7—39	n.p.	7—30	n.p.
	1178—1205
Coiled-coils	1060—1094	1065—1099	n.p.	1078—1108	n.p.	1066—1098	n.p.
N-glycosylation sites
Total number	9	5	6	7	3	11	7
Conserved position	905	910	895	921	912	909	891

Prediction of putative transmembrane helices, PEST sites, coiled-coils and N-glycosylation sites was performed with the programs TMHMM,PESTfind, PAIRCOIL and PROSCAN, respectively(Rogers et al., 1986; Berger et al., 1995, Bairoch et al., 1997; Hansen et al., 1997; Krogh et al., 2001).

pI, isoelectric point; TMH, transmembrane helices; n.p., not predicted.

This accession number refers to a sequence that reveals a truncated version of the protein. For calculations, the N-terminus was elongated according to 5′ upstream sequences found in the corresponding mosquito gene, which is located in region 7B of chromosome 2R

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