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Table 3.

Activity of citrate synthase, carnitine palmitoyl transferase and cytochrome c oxidase in mitochondrial suspensions, expressed in U mg⁻¹ mitochondrial protein

	Citrate synthase			Carnitine palmitoyl transferase
Step	5°C	15°C	5°C	15°C	5°C	15°C
Warm acclimation
Initial	0.74±0.05^c	1.02±0.03^c	0.014±0.002^a,b	0.019±0.001^c	1.37±0.09^a	1.73±0.09
Week 1	1.02±0.09^a	1.4±0.1^a	0.015±0.001^a,b	0.032±0.003^a,b	1.4±0.1^a,b	1.8±0.1
Week 2	0.98±0.04^a,b	1.54±0.05^a	0.0192±0.0009^a	0.036±0.001^a	1.8±0.1^a	2.1±0.1
Week 3	0.77±0.04^b,c	1.31±0.06^a,b	0.011±0.001^b	0.027±0.002^b	1.31±0.09^b	1.8±0.1
Week 4	0.64±0.04^c	1.05±0.06^b,c	0.012±0.002^b	0.026±0.003^a,b,c	1.4±0.1^a,b	1.6±0.2
Week 8	0.62±0.03^c	1.04±0.05^c	0.0121±0.0009^b	0.025±0.002^b,c	1.5±0.1^a,b	1.75±0.09
Cold acclimation
Initial	0.59±0.02^b	1.01±0.06	0.0094±0.0005^b	0.020±0.001^b	1.35±0.08^d	1.58±0.09^d
Week 1	0.59±0.03^b	1.01±0.05	0.0106±0.0005^b	0.022±0.001^b	1.6±0.2^c,d	1.8±0.2^c,d
Week 2	0.69±0.03^a	1.17±0.06	0.0139±0.0006^a	0.028±0.001^a	1.93±0.05^b,c	2.21±0.07^b,c
Week 6	0.73±0.03^a	1.20±0.04	0.0160±0.0005^a	0.027±0.001^a	2.40±0.09^a	2.8±0.1^a
Week 10	0.66±0.02^a,b	1.09±0.04	0.0153±0.0006^a	0.028±0.001^a	2.19±0.07^a,b	2.56±0.10^a,b

	Citrate synthase			Carnitine palmitoyl transferase
Step	5°C	15°C	5°C	15°C	5°C	15°C
Warm acclimation
Initial	0.74±0.05^c	1.02±0.03^c	0.014±0.002^a,b	0.019±0.001^c	1.37±0.09^a	1.73±0.09
Week 1	1.02±0.09^a	1.4±0.1^a	0.015±0.001^a,b	0.032±0.003^a,b	1.4±0.1^a,b	1.8±0.1
Week 2	0.98±0.04^a,b	1.54±0.05^a	0.0192±0.0009^a	0.036±0.001^a	1.8±0.1^a	2.1±0.1
Week 3	0.77±0.04^b,c	1.31±0.06^a,b	0.011±0.001^b	0.027±0.002^b	1.31±0.09^b	1.8±0.1
Week 4	0.64±0.04^c	1.05±0.06^b,c	0.012±0.002^b	0.026±0.003^a,b,c	1.4±0.1^a,b	1.6±0.2
Week 8	0.62±0.03^c	1.04±0.05^c	0.0121±0.0009^b	0.025±0.002^b,c	1.5±0.1^a,b	1.75±0.09
Cold acclimation
Initial	0.59±0.02^b	1.01±0.06	0.0094±0.0005^b	0.020±0.001^b	1.35±0.08^d	1.58±0.09^d
Week 1	0.59±0.03^b	1.01±0.05	0.0106±0.0005^b	0.022±0.001^b	1.6±0.2^c,d	1.8±0.2^c,d
Week 2	0.69±0.03^a	1.17±0.06	0.0139±0.0006^a	0.028±0.001^a	1.93±0.05^b,c	2.21±0.07^b,c
Week 6	0.73±0.03^a	1.20±0.04	0.0160±0.0005^a	0.027±0.001^a	2.40±0.09^a	2.8±0.1^a
Week 10	0.66±0.02^a,b	1.09±0.04	0.0153±0.0006^a	0.028±0.001^a	2.19±0.07^a,b	2.56±0.10^a,b

Data are means ± s.e.m. and are compared between acclimation steps within each experiment for each assay temperature. When values in a column are followed by different letters, they are significantly different (P≤0.05; ANOVA and a Tukey's a posterioritest).

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