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Table 2.

Toxicity to fourth instar Culex quinquefasciatus ofBacillus thuringiensis subsp. israelensis (Bti) strains that produce high levels of the B. sphaericus (Bs) binary toxin in comparison with wild-type Bti and Bs

Bacterial strainToxin compositionLC50 (ng ml−1)Improvementa
Bti IPS-82 + Bs Bin Cry4A + Cry4B + Cry11A + Cyt1A + Bs Bin 1.5±0.4 13× 
Bti 4Q7 + Bs Bin Bs Bin 1.4±0.4 10× 
Bti IPS-82 Cry4A + Cry4B + Cry11A + Cyt1A 19.5±3.5  
Bs 2362 Bs Bin 15.0±3.4  
Bacterial strainToxin compositionLC50 (ng ml−1)Improvementa
Bti IPS-82 + Bs Bin Cry4A + Cry4B + Cry11A + Cyt1A + Bs Bin 1.5±0.4 13× 
Bti 4Q7 + Bs Bin Bs Bin 1.4±0.4 10× 
Bti IPS-82 Cry4A + Cry4B + Cry11A + Cyt1A 19.5±3.5  
Bs 2362 Bs Bin 15.0±3.4  
a

Improvement represents the factor by which the toxicity per unit mass of lyophilized fermentation product is improved compared with that of the wild-type strains. The Bti IPS-82 + Bs Bin strain is compared with Bti IPS-82,a wild-type strain, and the Bti 4Q7 + Bs Bin strain is compared with Bs 2362,a wild-type strain.

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