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Table 2.

Comparison of branchial ammonia excretion rate calculated from Equation 4 to the measured net excretion rate from fish prior to (-0.5 h) and after 96 h exposure to CLP conditions

Time after addition of test water (h)
-0.5 96
Measured NH3 (μmol N kg-1 h-1)   137.2±16.4   342.1±21.8  
Δ Tamm(v̄—a) (μmol l-1)   69.8±12.8   82.8±16.7  
Cardiac output (ml min-1 kg-1)   34.5   45.8  
Calculated NH3 (μmol N kg-1 h-1)   144.2±24.7   227.6±51.8§ 
Calculated NH3/ Measured NH3  0.95±0.1   0.66±0.1  
Time after addition of test water (h)
-0.5 96
Measured NH3 (μmol N kg-1 h-1)   137.2±16.4   342.1±21.8  
Δ Tamm(v̄—a) (μmol l-1)   69.8±12.8   82.8±16.7  
Cardiac output (ml min-1 kg-1)   34.5   45.8  
Calculated NH3 (μmol N kg-1 h-1)   144.2±24.7   227.6±51.8§ 
Calculated NH3/ Measured NH3  0.95±0.1   0.66±0.1  

CLP conditions: pH 5, 0.08 μmol l-1 copper.

Mean value for cardiac output was the Routine value in Table 1.

§

indicates a significant difference between observed and expected values (χ2 test, d.f.=4, P=0.05, N=6).

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