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Table 1.

Measured apparent Km for exogenous ADP in different permeabilized cell preparations, and diffusion distances from the medium into the center of cells (Rdif)

PreparationMeasured diameter (μm)Rdif (μm)Apparent Km (ADP) (μmol 1-1)Reference
Cardiomyocytes 10-25 5-12.5 Opie, 1998  
 329±50 * Present study 
 16 250±38 Saks et al., 1991  
 20 10 200-250 Kay et al., 1997  
`Ghost' cardiomyocytes 20 10 200-250 Kay et al., 1997  
Skinned cardiac fibers 297±35 Saks et al., 1993  
 20 10 300±23 Saks et al., 2001  
 300-400 Seppet et al., 2001  
 370±70 Boudina et al., 2002  
 234±24 Liobikas et al., 2001  
 22 11 Menin et al., 2001  
 334±48 * Present study 
`Ghost' cardiac fibers 20 10 349±24 Saks et al., 1993  
 349±34 * Present study 
Skinned cardiac fibers + creatine 79±8 Saks et al., 1991  
 85±5 Kuznetsov et al., 1996  
Rat heart mitochondria in vitro ∼1 17.6±1 Saks et al., 1991  
PreparationMeasured diameter (μm)Rdif (μm)Apparent Km (ADP) (μmol 1-1)Reference
Cardiomyocytes 10-25 5-12.5 Opie, 1998  
 329±50 * Present study 
 16 250±38 Saks et al., 1991  
 20 10 200-250 Kay et al., 1997  
`Ghost' cardiomyocytes 20 10 200-250 Kay et al., 1997  
Skinned cardiac fibers 297±35 Saks et al., 1993  
 20 10 300±23 Saks et al., 2001  
 300-400 Seppet et al., 2001  
 370±70 Boudina et al., 2002  
 234±24 Liobikas et al., 2001  
 22 11 Menin et al., 2001  
 334±48 * Present study 
`Ghost' cardiac fibers 20 10 349±24 Saks et al., 1993  
 349±34 * Present study 
Skinned cardiac fibers + creatine 79±8 Saks et al., 1991  
 85±5 Kuznetsov et al., 1996  
Rat heart mitochondria in vitro ∼1 17.6±1 Saks et al., 1991  
*

Measured in this work for preparations similar to those described in Fig. 1. The diffusion distances are given to evaluate the rate of Brownian movement of ADP in water, if necessary (Saks et al.,2001).

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