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Table 1.

β-galactosidase activity in yeast cells with plasmids pAct-II,pAs-I or derivatives

his+,leu+ yeast plasmidsβ-galactosidase activity
pAct-II/pAs-1 0.004 
pAct-IIGpd-1/pAs-I 0.003 
pAct-IIGpd-3/pAs-I 0.002 
pAct-IIGpd-1/pAs-IGpd-3 0.521 
pAct-IIGpd-3/pAs-IGpd-1 0.485 
pAct-IIGpd-1/pAs-IGap 0.135 
pAct-IIGpd-3/pAs-IGap 0.582 
pAct-IIGpd-3/pAs-IPglym 0.903 
pAct-IIAld/pAs-IAld 0.631 
pAct-IIAld/pAs-IGpd-1 0.003 
pAct-IIAld/pAs-IGap 0.007 
his+,leu+ yeast plasmidsβ-galactosidase activity
pAct-II/pAs-1 0.004 
pAct-IIGpd-1/pAs-I 0.003 
pAct-IIGpd-3/pAs-I 0.002 
pAct-IIGpd-1/pAs-IGpd-3 0.521 
pAct-IIGpd-3/pAs-IGpd-1 0.485 
pAct-IIGpd-1/pAs-IGap 0.135 
pAct-IIGpd-3/pAs-IGap 0.582 
pAct-IIGpd-3/pAs-IPglym 0.903 
pAct-IIAld/pAs-IAld 0.631 
pAct-IIAld/pAs-IGpd-1 0.003 
pAct-IIAld/pAs-IGap 0.007 

Abbreviations: Gpd, glycerol-3-phosphate dehydrogenase; Gap,glyceraldehyde-3-phosphate dehydrogenase; Pglym, phosphoglycerol mutase; Ald,aldolase.

β-galactosidase activity is expressed in fluorescence units per mg protein in extracts of yeast cells. Each value is the mean of three determinations.

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